HER2 expression in tumors after in vivo growth of MC38-hHER2-Luc in wild type C57BL/6 mice. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

2023年8月24日 · HER2-overexpressed cell lines (MC38 HER2, CT26 HER2) were generated using lentivirus transfection and HER2 was successfully overexpressed and located on the cell membrane, compared to...

B-hHER2 MC38 cells were subcutaneously transplanted into heterozygous B-hCD3E/hHER2 mice (female, 7-week-old, n=5), and on 31 days post inoculation, tumor cells were harvested and assessed for human HER2 expression by flow cytometry.

2024年1月2日 · HER2-transduced MC38 mouse colon cancer cells (MC38-HER2), CD19-expressing PANC1 epithelioid cells (PANC1-CD19) and CD19-expressing NIH/3T3 cells (NIH/3T3-CD19) were generated according to ...

2024年9月25日，Nature 期刊同期发布两篇重磅研究，揭示了Type-2免疫在维持经CAR-T治疗的B细胞急性淋巴白血病（B-ALL）患者超 8年无癌临床反应的关键作用，以及Type-2细胞因子Fc–IL-4协同Type-1免疫疗法高效、持久清除多种实体肿瘤的内在免疫学和分子生物学机制。 这两项研究分别聚焦患者长期临床追踪（超10年）和临床前机理研究，得出了一致且相互支持的结论，即Type-2免疫可能在维持长效持久的抗癌免疫反应中起关键作用。 这一发现揭示了Type-2免疫反 …

2024年9月26日 · 比如，在黑色素肿瘤YUMM1.7-OVA模型中，Fc–IL-4联合OT1细胞疗法实现了100%的肿瘤清除；在结肠癌MC38-HER2模型中，Fc–IL-4联合HER2-CAR-T细胞疗法实现了87%的肿瘤清除；在Raji人淋巴瘤的NSG小鼠模型中， 人源的Fc–IL-4联合CD19-CAR-T细胞疗法清除了75%的肿瘤。 除此之外，在结肠癌MC38模型中，Fc–IL-4与ICB联用实现了100%的肿瘤清 …

2021年5月14日 · In this study we developed a bispecific antibody (BsAb) simultaneously targeting both PD1 and HER2 in an attempt to combine HER2-targeted therapy with immune checkpoint blockade for treating...

In this study, the mouse HER2 gene was replaced with the human HER2 coding sequence in B-hHER2 CT26.WT cells and B-hHER2 MC38 cells. Several HER2-targeting antibody drugs and antibody-drug conjugates (ADCs), including trastuzumab, pertuzumab, T-DM1, DS8201, and RC48, have been approved worldwide for patients with HER2-positive tumors.

在预先建立的mc38-her2肿瘤治疗环境中，il-10 her2 car-t细胞过继在90%的治疗小鼠中诱导实体肿瘤完全消退和持久治愈(图5a-c)。 相比之下，HER2 CAR-T细胞单独或联合单次或多次静脉注射本地IL-10可暂时控制肿瘤生长，但未能诱导任何持久的肿瘤消退。

2018年10月12日 · 125 I-Herceptin has a high affinity for HER2 in MC38 colon cancer cells. SPECT imaging of HER2 in colon cancer using 125 I-Herceptin is feasibility. An effective, non-invasive and less costly in vivo detection method of HER2.