丙二醛 (MDA)是常用的膜脂过氧化指标，在较高温度（95度）及酸性环境中可与硫代巴比妥酸 (thiobarbituric acid，TBA)缩合，形成红色的MDA-TBA加合物，相应的反应原理图，如下：

Malondialdehyde (MDA) is one of the final products of polyunsaturated fatty acids peroxidation in the cells. An increase in free radicals causes overproduction of MDA. Malondialdehyde level is commonly known as a marker of oxidative stress and the antioxidant status in cancerous patients.

Malondialdehyde belong to the class of β-dicarbonyls. A colorless liquid, malondialdehyde is a highly reactive compound that occurs as the enol. [2] . It is a physiological metabolite, and a marker for oxidative stress. Malondialdehyde mainly exists as its enol, hydroxyacrolein: [2]

To study the level of serum malondialdehyde (MDA), a biomarker of oxidative stress before and after chemotherapy in various ocular malignancies and to correlate its significance with clinicopathological parameters. Thirty two histopathologically confirmed cases of primary ocular malignancies were included in this longitudinal cohort study.

为了测量和分析氧化应激，研究人员依靠一系列“生物标志物”，如丙二醛 （MDA）、8-羟基-2'-脱氧鸟苷 （8-OHdG） 和 谷胱甘肽（Glutathione）、 超氧化物歧化酶 （SOD）、核因子红细胞 2 相关因子 2 （Nrf2）、白藜芦醇（Resveratrol）等。

2022年5月1日 · Malondialdehyde (MDA) is one of radical oxidative marker, while Superoxide Dismutase (SOD) play role as endogenous antioxidant. It has been postulated that in cancer cells there is an increase of oxidative stress compared to normal cell.

Malondialdehyde (MDA) is the most frequently used biomarker of oxidative stress in many health problems such as cancer, psychiatry, chronic obstructive pulmonary disease, asthma, or cardiovascular diseases. This perspective study aimed to collect some evidence for low reliability of the MDA as a biomarker of oxidative stress.

2021年6月7日 · 超氧化物歧化酶（SOD）和丙二醛（MDA）是评价氧化应激过程中使用最多的两个指标。 Elabscienc®研发出2款样本用量更少、适合大批量测定的SOD和MDA试剂盒，这2款试剂盒分别基于WST-1法和TBA法而研发的。

2020年9月1日 · The level of oxidative stress can be measured by assessing the MDA levels. This study aimed to evaluate serum MDA levels in patients with bladder cancer, as well as to determine its potential role as a biomarker in the diagnosis of …

2023年9月12日 · MDA Level Measurement：To determine the generation of MDA as the metabolite of lipid peroxidation of MDR E. coli, an MDA Content Assay Kit (BC0025, Solarbio) was utilized following the instructions provided by the manufacturer.