2025年2月25日 · Here, we revealed that RNF20 mediates the classical K48 and non-classical K27-linked ubiquitination and degradation of chicken MDA5, thereby preventing excessive immune responses. Notably, human RNF20 can suppress IFN-γ-induced IRF1 transcription by modulating the phosphorylation of the C-terminal domain (CTD) of RNA polymerase II ( 35 ...

2024年9月16日 · MDA5 is a critical cytoplasmic RNA sensor capable of triggering type I interferon pathways. This study shows that the E3 ubiquitin ligase RNF144B suppresses innate antiviral responses via ubiquitin...

2021年11月5日 · 近期中国科学院上海药物研究所郑杰团队在Immunity杂志上以Research Article形式在线发表了题为“Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains”的研究成果，本研究通过生物大分子氢氘交换质谱技术（HDX-MS ...

2020年9月2日 · Parkin directly interacted with and catalyzed the K48-linked polyubiquitination and subsequent degradation of RIG-I and MDA5. Thus, we demonstrate that Parkin limits RLR-triggered innate immunity activation, suggesting Parkin as a potential therapeutic target for the control of viral infection.

2023年11月13日 · In the present study, we reveal that TRIM4 positively regulates MDA5–mediated antiviral immune responses by inducing MDA5 K63-linked polyubiquitination at K43 and K174, depletion of TRIM4 or mutation of MDA5 at both sites greatly suppresses MDA5 K63-polyubiquitination and antiviral innate immune responses.

2017年11月7日 · In this study, we identified a major histocompatibility complex (MHC)-encoded gene, tripartite interaction motif 40 (TRIM40), as a suppressor of RLR signaling by directly targeting MDA5 and RIG-I. TRIM40 binds to MDA5 and RIG-I and promotes their K27- and K48-linked polyubiquitination via its E3 ligase activity, leading to their proteasomal ...

2024年10月23日 · usp7可与mda5相互作用，通过抑制mda5的k48位泛素化抑制mda5降解。 结论：USP7通过抑制MDA5的K48位泛素化修饰稳定MDA5表达，从而正向调控EV71感染免疫和炎症反应。

2020年9月1日 · It has been identified that several E3 ligases promote K48-linked ubiquitination of RIG-I and MDA5, including RNF125, Siglec-G, TRIM40, and STUB1 (53–56). Here, we demonstrate that Parkin served as a specific regulator of RIG-I-mediated and MDA5-mediated innate antiviral immunity through direct conjugation of K48-linked polyubiquitin chains ...

In this study, we identified a major histocompatibility complex (MHC)-encoded gene, tripartite interaction motif 40 (TRIM40), as a suppressor of RLR signaling by directly targeting MDA5 and RIG-I. TRIM40 binds to MDA5 and RIG-I and promotes their K27- and K48-linked polyubiquitination via its E3 ligase activity, leading to their proteasomal degr...

2021年1月18日 · The zinc finger FYVE domain-containing protein ZFYVE1 specifically associates with MDA5 and inhibits MDA5-mediated signaling. 300 FK506-binding protein 8 (FKBP8) inhibits the RLR-MAVS pathway...