Anti-Melanoma Differentiation-Associated gene 5 (MDA-5) Dermatomyositis (MDA5, DM) is a recently identified subtype of myositis characteristically associated with Rapidly Progressive Interstitial Lung Disease (RP-ILD) and unique cutaneous features.

2024年6月6日 · In this study, we elucidated the intricate interplay between the chaperon protein 14-3-3η, the cytosolic RNA sensor MDA5, and the protease Caspase-3. Upon virus infection, MDA5 activation triggers Caspase-3 activity, leading to the cleavage of 14-3-3η and the formation of a truncated version termed sub-14-3-3η.

2023年1月26日 · Anti-melanoma differentiation-associated gene 5 (MDA 5) is one of the subtypes of dermatomyositis associated with rapidly progressive lung disease. MDA 5 carries a high mortality risk due to respiratory failure.

The KD RIG-I, KD MDA5, dKD and the parental NIH 3T3 cells were infected with VEEV/GFP/Cm and SINV/G/GFP viruses. The results presented in Fig. 3A demonstrate that upon infection, both single KD cell lines (KD RIG-I and KD MDA5) were still capable of IFN-β expression to levels comparable to those detected in infected NIH 3T3 cells.

2024年1月2日 · The results showed that MDA5 knockdown reduced IFN-λ1 and IFN-λ2/3 production and increased vRNA expression in EV-D68-infected Calu-3 cells (Fig 5 B). Furthermore, the titer of EV-D68 was also elevated in MDA5-KD cells (Fig 5 C). To confirm whether IRF3 and IRF7 are involved in MDA5-mediated IFN-λ …

MDA5 ATP hydrolysis is strictly dependent on dsRNA stimulation and displays greater ATPase activation by longer dsRNA substrates. In the presence of optimal dsRNA substrates, MDA5 exhibits a lower rate of ATP hydrolysis than RIG-I [63].

MDA5 regulates inflammatory signals, whereas PTPN2 may function as a defence mechanism against pro-apoptotic signals generated by dsRNA. These two candidate genes for T1D may thus modulate β-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic β-cell level.

2021年11月5日 · mda5是细胞内的异体rna监测蛋白，属于rig-i样受体家族（rlrs）的重要成员。 MDA5参与多种RNA病毒引起的免疫反应，是天然免疫的一道重要屏障。 RLRs家族共有RIG-I、MDA5及LGP2三个成员，其中RIG-I和MDA5的N端均拥有串联CARDs结构域，可通过CARD-CARD同型相互作用招募MAVS ...

2023年11月3日 · Anti-MDA5 (Melanoma differentiation-associated protein-5) positive dermatomyositis (MDA5 + -DM) is characterised by rapidly progressive interstitial lung disease (ILD) and high mortality. MDA5 is an RNA sensor and a key pattern recognition receptor for the SARS-CoV-2 virus.

2024年10月3日 · Many MDA5 variants are associated with protection from autoimmune disease, while increasing the risk of infection and chronic inflammation, but how these variants affect MDA5-dependent RNA sensing remains unclear. Here, we determine the consequences of autoimmune-protective MDA5 variants on the molecular structure and activities of MDA5.