2024年10月3日 · MDA5-dependent interferon production and inflammation following viral infection can trigger autoimmune b-cell killing. Here, we examine the consequences of T1D-protective MDA5 substitutions on the structure and activities of MDA5. We show that variants E627*, I923V, and T946A reduce the IFN-b response to picornavirus infection.

2024年4月1日 · Melanoma differentiation-associated protein 5 (MDA5), encoded by interferon-induced with helicase C domain 1 (IFIH1), is a cytoplasmic sensor that primarily functions as a detector of double-stranded ribonucleic acid (dsRNA) viruses in innate immune responses; however, its role in CH pathogenesis remains unclear.

2024年5月16日 · Mechanistically, ADAM9 binds to MDA5 and promotes its oligomerization and thereby downstream mitochondrial antiviral-signaling protein (MAVS) activation in response to EMCV RNA stimulation.

2021年1月18日 · RNA recognition by MDA5. An analysis of MDA5-KO mice revealed that MDA5 is involved in detecting viruses belonging to the Picornaviridae family, including EMCV and Theiler’s encephalomyelitis...

Remarkably, ADAR1 knockout neuronal progenitor cells exhibited MDA5 (dsRNA sensor)-dependent spontaneous interferon production, PKR activation, and cell death. Thus, human ADAR1 regulates sensing of self versus nonself RNA, allowing pathogen detection while avoiding autoinflammation.

THP1-Dual™ KO-MAVS cells feature the knockout of the MDA5 gene. They express two inducible reporter genes, allowing the study of the IRF and NF-κB pathways.

RAW-Lucia ™ ISG-KO-MDA5 cells were generated from RAW-Lucia ™ ISG cells through the stable knockout of the MDA-5 gene. These cells derive from the murine RAW 264.7 macrophage cell line, which has been reported to express many pattern recognition receptors (PRRs), including the dsRNA sensors MDA-5 [1] and RIG-I [2,3] along with their adaptor ...

2021年3月16日 · Activation of the RIG-I-like receptors, retinoic-acid inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5), establishes an antiviral state by upregulating interferon...

InvivoGen A549-Dual™ KO-MDA5 Cells are A549 human reporter cells designed for cell-based assays of MDA5 (IFIH1) function through monitoring of IFN and NF-kB pathway induction.

Utilizing nonobese diabetic (NOD) mice lacking Ifih1 expression (KO) or containing an in-frame deletion within the ATPase site of the helicase 1 domain of MDA5 (ΔHel1), we tested the hypothesis that partial or complete loss-of-function mutations in MDA5 would delay T1D by impairing proinflammatory pancreatic macrophage and T cell responses.