MDA5 (melanoma differentiation-associated protein 5) is a RIG-I-like receptor dsRNA helicase enzyme that is encoded by the IFIH1 gene in humans. [5] MDA5 is part of the RIG-I-like …

InvivoGen RLR ligands are agonists of RIG-I and/or MDA-5. Each lot is functionally tested on cellular assays. The most popular agonists are triphosphate RNA (pppRNA) and ready-to-use …

2020年3月13日 · In contrast to RIG-I, MDA5 is believed to adopt a more open conformation in the absence of RNA ligand. In the presence of long double-stranded RNA (dsRNA), MDA5 …

Here, we show that MDA5 cooperatively binds short RNA ligands as a dimer with a 16–18-basepair footprint. A crystal structure of the MDA5 helicase-insert domain demonstrates an …

RIG-I recognizes short RNA ligands with 5′-triphosphate caps. MDA5 recognizes long kilobase-scale genomic RNA and replication intermediates. Ligand binding induces conformational …

2014年4月17日 · While it is well established that RIG-I binds 5′-triphosphate containing double-stranded RNA, the in vivo occurring ligand for MDA5 is poorly characterized. A major …

Prior to RNA recognition, ligand-free MDA5 is present in the cytoplasm (Fig. 2 F). When foreign cytoplasmic RNA is detected, MDA5 binds initially as a monomer to dsRNA (Fig. 2 G), …

2012年11月11日 · The viral dsRNA, RF, has been assumed to be the MDA5 ligand produced by picornaviruses based on the fact that all known activators of MDA5 are dsRNAs (mimics). …

2014年4月17日 · RIG-I-like receptors (RLRs: RIG-I, MDA5 and LGP2) play a major role in the innate immune response against viral infections and detect patterns on viral RNA molecules …

2018年12月31日 · Upon ligand recognition and binding, RIG-I and MDA5 undergo a conformational change that renders the CARD domains available for homotypic interaction …