2017年9月25日 · In SAM, mouse embryonic fibroblast (MEF) growth were fully inhibited by 10 μg/ml mitomycin-C (MMC) in 0.5 hours, and the feeder cells generated display higher adherent and recovery rates as...

2018年5月17日 · Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we demonstrated a novel method to...

2023年1月4日 · Human induced pluripotent stem cells were seeded on ethanol‐fixed MEF (ET‐MEF) cells, and mitomycin C‐treated HDF (MMC‐HDF) cells. The results showed that the morphology of hiPS cells cultured on ET‐MEF was similar to that cultured on MMC‐HDF, presenting the flat and domed cell types (Fig. S1A ).

MEF细胞 的培养基. 培养基： DMEM-H +10% FBS +双抗. 二． MEF的解冻. 1. 从液氮中取出一支冻存的细胞，迅速将其投入37度水浴锅中，震荡，使其尽快溶解； 2. 迅速将冻存管转移至超净台中，喷75%酒精消毒； 3. 用吸管将细胞转移至装有9mL培养基的离心管中，轻柔的混匀 ...

2012年3月2日 · Mitomycin-C (MMC)-treated MEF feeder cells were prepared by incubating MEFs with DMEM medium supplemented with 10% FBS and 10 µg/mL of MMC at 37°C for 3–4 h. The medium was then changed to DMEM for miPS cells. MMC-treated feeder cells were incubated overnight at 37°C before use.

小鼠胚胎成纤维细胞(mef) 作为原代培养细胞，其刺激增殖的能力强且可靠、易培养、来源丰富，常被用作干细胞培养的饲养层。 一方面提供刺激干细胞增殖的各种因子，另一方面保持干细胞的未分化状态。

2013年5月27日 · mmc作用不是灭活，只是抑制MEF的分裂而已，但MEF细胞仍然会长大变长。 若MEF不经过mmc处理，直接培养ips的话，得出的结果是MEF生长速度快于ips，最后MEF完全占据培养空间，ips不会养的好

2009年7月1日 · Mitomycin C (MMC) is used to inactivate mEFs, but this causes DNA damage, and it is unclear whether MMC remains in the culture system after several washes. Three variables have been evaluated with respect to feeder preparation and MMC involvement, including mEF exposure to MMC, density of feeder cells , and different wash steps during the ...

2018年5月17日 · Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we demonstrated a novel method to prepare feeder cells with high efficiency, timesaving, and low costs. MEFs in 3 × 10 4 cell/cm 2 were fixed by methanol for 5 min and air drying for 5 min.

Treatment of MEFs with Mitomycin (MMC) MEF cell density for use as an MMC-treated feeder cell layer is 2.5 × 10 6 cells per 10 cm dish. Treat MEF cells with MMC (10 µg/mL in MEF medium) and incubate at 37°C for 2 to 3 h to inhibit further proliferation.