2021年6月7日 · The assays of homologous recombination repair, mitomycin C (MMC) sensitivity, immunofluorescence, and ubiquitination modification were performed in U2OS and DR-U2OS cell lines. In MMC-treated U2OS cells, the downregulation of the DNAH2 gene increased the sensitivity of cells to DNA inter-strand crosslinks.

2013年10月4日 · Ouabain enhances cytotoxicity of MMC against U2OS cells. (A) U2OS cells were pretreated with the 50 nM ouabain for 1 h and then treated with 2000 ng/ml MMC. After incubation for 48 h, cell viability was measured with the MTT assay. Values represent the means ± SEM from three independent experiments (Student’s t-test, ***, P < 0.001). (B ...

2013年9月4日 · Genetic disruption of HELQ in human cells enhances cellular sensitivity and chromosome radial formation by the ICL-inducing agent mitomycin C (MMC). A significant fraction of MMC sensitivity is...

在细胞 分级分离 实验中，作者发现mmc处理的u2os细胞沉淀（含染色质）中s556、s559和s565的磷酸化显著增加。这表明大多数fanci磷酸化发生在与染色质结合的fanci上。

2024年2月7日 · U2OS cells lacking MutSβ were shown to accumulate G4 structures and R-loops not only at telomeres but also at nontelomeric loci (15), suggesting a genome-wide role for MutSβ in the regulation of these aberrant structures.

Rapidly dividing cancer cells are hypersensitive to the DNA damaging effects of cisplatin, psoralen, mitomycin C (MMC) and other DNA cross-linking drugs; however, the cytotoxicity of such compounds for normal (noncancerous) cells has posed a significant drawback for their efficacy in combating cancer.

2016年1月21日 · Defects in repairing ICLs are implicated in Fanconi anemia (FA), a rare autosomal recessive disease typified by hypersensitivity to ICL-inducing agents such as mitomycin-C (MMC) or diepoxybutane (DEB), exaggerated G2 arrest, and increased chromosome abnormalities after exposure to these agents .

(A-B) SIRF assay to examine the presence of FANCM (A) and FAAP24 (B) at ICL-stalled replication forks in indicated U2OS cells treated with DMSO or MMC. Right panel, quantification of FANCM or FAAP24 signal.

2017年6月1日 · RFWD3 inhibits persistent mitomycin C (MMC)-induced RAD51 and RPA foci by promoting VCP/p97-mediated protein dynamics and subsequent degradation. Furthermore, MMC-induced chromatin loading of MCM8 and RAD54 is defective in cells with inactivated RFWD3 or expressing a ubiquitination-deficient mutant RAD51.

We utilized the siRNA approach to down-regulate the expression level of DNAH2 and FANCA (as positive control) in U2OS cell line. The mRNA level of DNAH2 or FANCA was significantly decreased upon...