
Laboratory Testing for Methicillin (oxacillin)-resistant
2024年4月12日 · Phenotypic methods recommended for the detection of MRSA include: Cefoxitin broth microdilution. Oxacillin broth microdilution. Cefoxitin disk diffusion testing. A screening …
Rapid PCR-Based Identification of Methicillin-Resistant
A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA …
Rapid screening and identification of methicillin-resistant ...
A screening method for methicillin-resistant Staphylococcus aureus (MRSA) by using selective broth and real-time PCR (broth-PCR) was developed and evaluated. The samples (n = 304) …
Use of broth enrichment and real-time PCR to exclude the …
2004年8月13日 · A rapid and sensitive method for excluding the presence of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples was developed and evaluated. The …
Evaluation of a duplex real-time PCR assay to detect MRSA from broth …
2013年11月11日 · This study evaluated a quantitative real-time PCR assay targeting mecA (encoding high level resistance to methicillin) and femB (a specific genomic marker for S. …
Evaluation of the IDI-MRSA Assay for Detection of Methicillin …
By this approach, a quantitative real-time PCR assay for detection of nuc is required to differentiate the growth of MRSA from that of MSSA from clinical specimens pooled in a …
A PCR method for the identification of methicillin-resistant ...
The PCR method involved pre-incubation in broth for 4 hours followed by a multiplex PCR with primers directed to mecA and nuc genes of MRSA. The reference standard was determined by …
A Rapid and High-Throughput Screening Approach for Methicillin ...
We developed a rapid and high-throughput molecular screening approach that consists of an overnight selective broth enrichment, followed by mecA, mecC, and S. aureus-specific (SA442 …
A rapid and sensitive method for excluding the presence of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples was developed and evaluated. The method utilised an …
Detection of Methicillin-Resistant Staphylococcus aureus …
We describe a qualitative in vitro diagnostic test for the rapid detection of MRSA directly from nasal swab specimens (IDI-MRSA; Infectio Diagnostic, Inc., Sainte-Foy, Québec, Canada), …