Analysis of post-translational modifications (PTMs) by mass spectrometry can be difficult and the level of difficulty is dependent on (i) the mass shift in the peptide molecular weight, (ii) the overall abundance of the modified peptide, (iii) the stability of the modification during mass spectrometry (MS) and MS/MS analysis, and (iv) the ...

蛋白质翻译后修饰 (post-translational modifications, PTMs) 是指在翻译后的蛋白质氨基酸侧链及其C/N末端引入新的基团 (如磷酸基、乙酰基等)。 PTM在细胞分化、蛋白质降解、信号传导和调节、基因表达调节以及蛋白质互作过程中起着关键作用。 液相色谱-质谱联用 (LC-MS/MS)技术是进行蛋白质修饰鉴定的首选技术，可以同时实现 修饰类型 、 修饰位点 的鉴定以及 修饰的相对定量。 本文主要介绍基于质谱的蛋白质翻译后修饰鉴定原理，并分析修饰鉴定和修饰组学产品的区别以 …

2007年9月27日 · We describe MS strategies used to detect common PTMs, map modified residues and estimate stoichiometries, with particular focus on protein phosphorylation as a well developed example. We also...

2023年7月12日 · The PTM-Shepherd diagnostic feature mining module aims to perform high throughput identification of spectral features that can be used to identify post-translational modifications (PTMs ...

2014年12月26日 · Here, we provide an update of MS-based PTM analyses, with a focus on current enrichment strategies coupled with revolutionary advances in high performance MS. Furthermore, we discuss examples of the discovery of recently described biological roles of PTMs and address the challenges of defining site-specific functions.

2022年8月1日 · Post-translational modifications (PTMs) induce marginal chemical additions to native proteins and lipoproteins after their biosynthesis, generating various isoforms with altered biological functions, PTMs are covalent chemical modifications of proteins, peptides, and lipoproteins after their biosynthesis by low molecular weight molecules.

2019年11月1日 · Protein PTM site stoichiometry is the fraction of proteins that contain a specific PTM at a given site. Thorough understanding of PTM site stoichiometry is valuable for revealing global properties of the different types of PTMs, and for understanding the mechanistic implications of PTMs in regulating protein function.

2022年11月1日 · 串联质谱分析是指在一级质谱之后选择特定 m/z 的 母离子 进行二级 MS 分析，可以确定肽段的氨基酸序列以及翻译后修饰的类型和位点。 蛋白酶实质上是一种具有催化作用的蛋白质，在很多生理化学反应中起着重要的桥梁作用。 蛋白酶作为一种蛋白质，在合成过程中也会发生各种翻译后修饰，如磷酸化、甲基化、乙酰化、泛素化以及糖基化等。 这一系列修饰通…

对于药物开发、突变分析和激酶分析研究，CST 科学家颠覆了传统的抗体开发过程并设计出一种至今仍然推动重要发现的全新 PTM 检测方法：PTMScan。 检测 PTM 的新方法：什么是 PTMScan？

2021年1月1日 · PTM crosstalk describes the combinatorial action of multiple PTMs on the same or on different proteins for higher order regulation. Here we review how recent advances in proteomic technologies, mass spectrometry instrumentation, and bioinformatics spurred the proteome-wide identification of PTM crosstalk through measurements of PTM sites.