干细胞（mouse BM MSCs，mBMMSCs）通过细胞与细胞 间接触来上调PD-L1 的表达，再通过PD-1/PD-L1 通路对 成熟辅助性T（T helper，Th）17 细胞起选择性抑制作用。 MSCs 免疫调节功能的机制已被广泛研究，但尚未完 全阐明。 目前明确的是MSCs 主要通过两种途径发挥免疫 抑制功能： （1）可溶性细胞因子的分泌，如转化生长因子（TGF）-β1、肝细胞生长因子（hepatocyte growth factor， HGF）、前列腺素E2（prostaglandin E2，PGE2）和一氧化氮。 （2）细胞与 …

2023年12月18日 · PD-L1 expression levels of WJ-MSCs could be regarded as an indicator to choose optimal MSCs for treating autoimmune disease. These findings provided novel insights into the quality control of MSCs and will inform improvements in the therapeutic benefits of MSCs.

2018年8月26日 · MSCs are identified in various adult organs including dental tissues. Periodontal ligament (PDL) is a highly specialized connective tissue that surrounds the tooth root. PDL also contains MSC population, and many researchers have isolated them and performed their detailed characterization.

In TME, mesenchymal stem cells (MSCs) play a crucial role in tumor progression, metastasis, and drug resistance. Emerging evidence suggests that MSCs can modulate the immune-suppression capacity of TME through the stimulation of PD-L1 expression in various cancers; nonetheless, their role in the induction of PD-L1 in breast cancer remained elusive.

Periodontal ligament stem cells (PDLSCs), which reside in the perivascular space of the periodontium, possess characteristics of mesenchymal stem cells and are a promising tool for periodontal regeneration. Recently, great progress has been made in PDLSC transplantation.

2023年5月5日 · 建议以群体倍增水平（ pdl ）定义细胞代次，或明确细胞传代过程中的群体倍增时间（ pdt ）以开展研究。 传代稳定性研究的条件应能代表实际临床/商业化生产工艺，重点关注遗传稳定性、成瘤/致瘤性、细胞干性、多能性、目的细胞分化能力等，并根据研究制定 ...

尽管来自脱落乳牙（SHED），PDL-MSC和AT-MSC的MSC显示多能性标记的mRNA表达更高，但是所有MSC均表达多能性标记蛋白。 SHEDs和PDL-MSCs具有显着的成骨能力，PB-MSCs和UC-MSCs具有增强的成脂分化潜能，而AT-MSCs表现出相似的分化能力。 意义尽管显示相似的表型，但来自不同来源的MSCs群体，在与其自我更新和分化能力有关的生物学特性方面表现出高度的可变性。 这些数据表明，为了更准确地用于细胞治疗，应确定从不同组织分离的MSC的个 …

2021年9月29日 · Adapted from Best Practices in MSC Culture: Tracking & Reporting Cellular Age Using Population Doubling Level & Not Cell Passage Number. Is it impolite to ask an MSC its real cell age? Of course not—and your research program will thank you. Why? It’s well known that cell phenotype and biological function can change as cells replicate in ...

2019年4月15日 · Our findings demonstrated for the first time that MSC exosomes enhance periodontal regeneration possibly by increasing PDL migration and proliferation. This study suggests that MSC exosome is a viable ready-to-use and cell-free MSC therapeutic for the treatment of periodontal defects.

整体计算：PDL（P5）=PDL（P0）+log2 (N5末/N1始) 。 注：“Nx始”为该步骤接种时的细胞数量，“Nx末”为该步骤培养结束时的细胞数量，“PDL（P0）”为工作细胞的PDL代次。