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微量热涌动 （MicroScale Thermophoresis，MST）技术是一种基于检测在温度梯度中的生物分子电泳迁移率的变化而检测生物分子间结合、解离过程，获取分子间相互作用的模式和动力学常数等方面信息的新技术[1]，广泛用于预防医学与公共卫生学、基础医学、临床医学 ...

To obtain ΔRatio (Spectral Shift) or ΔFnorm (MST), the baseline value is subtracted from all data points of the same curve. Thus, by definition, ΔRatio or ΔFnorm is 0 in the unbound state and can adopt positive or negative values, depending on the direction of the response amplitude.

2014年12月5日 · MicroScale Thermophoresis (MST) measures dissociation constants down to 1 picomolar. MST can dissect different binding mechanism by distinct thermophoresis signals. MST quantifies dissociation constants in 100% cell lysate. MST can be used to determine thermodynamic parameters for interactions.

微量热泳动技术 (Microscale Thermophoresis，MST) 是一种定量分析生物分子间相互作用的前沿技术，通过精确检测荧光变化，结合灵敏的热泳动现象，定量分析分子间相互作用。

2017年12月15日 · Microscale thermophoresis (MST) is a powerful new method for the quantitative analysis of protein-protein interactions (PPIs) with low sample consumption. In...

2024年12月10日 · MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge, or size.

MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in …

mst 技术是一种基于检测在温度梯度中的生物分子电泳迁移率的变化而检测生物分子间结合、解离过程，获取分子间相互作用的模式和动力学常数等方面信息的新技术，是近年来发展的研究生物分子相互作用的强有力工具，已广泛应用于生命科学领域研究。

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