In this work, we focus on the methods to analyze one sub-type of microautophagy that mediates degradation of cytosolic proteins in late endosome/multi-vesicular bodies (LE/MVB), which is known as endosomal microautophagy (eMI) (Sahu, Kaushik et al., 2011). eMI can take place “in bulk” or selectively upon binding of hsc70, like in CMA, to ...

2023年12月6日 · In endosomal microautophagy (eMI), 18 the subtype of microautophagy with the highest similarity to CMA, substrates are also targeted for degradation upon recognition of a KFERQ-like motif by Hsc70. 18 However, in eMI, Hsc70 binds to phosphatidylserine in the LE/MVB membrane, triggering the loading of substrate into vesicles that form on the ...

2018年4月13日 · Mammalian eMI originated from studies attempting to characterize the contribution of autophagic pathways to antigen presentation in dendritic cells. Analysis of LE/MVB, where cytosolic antigens are processed for presentation, revealed that arrival of some cytosolic proteins persisted even upon genetic blockage of macroautophagy or CMA (8 ...

In this work, we show the existence of a compensatory relationship between CMA and eMI and identify a role for the chaperone pro-tein Bag6 in triage and internalization of eMI substrates into late endosomes.

Endosomal microautophagy (eMI) is a type of autophagy that allows for the selective uptake and degradation of cytosolic proteins in late endosome/multi-vesicular bodies (LE/MVB). This process starts with the recognition of a pentapeptide amino acid KFERQ-like targeting motif in the substrate protein …

Chaperone-mediated autophagy (CMA) and endosomal microautophagy (eMI) are pathways for selective degradation of cytosolic proteins in lysosomes and late endosomes, respectively. These autophagic processes share as a first step the recognition of the ...

2020年7月9日 · In contrast to CMA, eMI is dependent on MVB formation for uptake of substrates into late endosomes in mammalian cells and in Drosophila [24, 26, 27]. Similar to starvation-induced eMI, sensor puncta formation upon DNA damage and oxidative stress is dependent on members of the ESCRT machinery.

2021年1月1日 · In this work, we focus on the methods to analyze one sub-type of microautophagy that mediates degradation of cytosolic proteins in late endosome/multi-vesicular bodies (LE/MVB), which is known as endosomal microautophagy (eMI) (Sahu et al., 2011). eMI can take place “in bulk” or selectively upon binding of hsc70, like in CMA, to the KFERQ ...

Chaperone-mediated autophagy (CMA) and endosomal microautophagy (eMI) are pathways for selective degradation of cytosolic proteins in lysosomes and late endosomes, respectively.

During endosomal microautophagy (eMI), cytoplasmic proteins are engulfed into late endosomes in an ESCRT machinery-dependent manner. eMI can be KFERQ-specific or occur in bulk and be induced by prolonged starvation. Its physiological regulation and function, however, are …