In the present study, the effects of the MYC point mutation T58A on the progression of a cellular tumor antigen p53 (p53) −/− human breast cancer cell line was analyzed, and the mechanism of p53-independent MYC-induced apoptosis was investigated. HCC1937 cells were transfected with mutant (T58A) or wild-type (WT) MYC using lentiviral vectors.

2012年4月23日 · To examine Myc dependence upon Ras mutations we have generated MMTV regulated Myc and Myc T58A transgenic mice. Expression of the more stable T58A Myc allele resulted in a reduction in...

2020年9月7日 · In the T58A mutant, MYC is stabilized with constitutive S62 phosphorylation. When challenged with DMBA/TPA-mediated carcinogenesis, MYC T58A mice had accelerated development of papillomas,...

2013年3月7日 · Expression of the more stable T58A Myc allele resulted in a reduction in KRas-activating mutations. However, in a low-level expression T58A Myc transgenic, the majority of the tumors were squamous or epithelial-to-mesenchymal in nature and accumulated KRas mutations at a higher frequency.

2022年4月5日 · MYC 的 T58 到丙氨酸突变体 （T58A） 模拟稳定的 S62 磷酸化和高致癌形式的 MYC。 S62A 突变体也是稳定的，在丝氨酸 62 和苏氨酸 58 位点均缺乏磷酸化，并且已被证明在体外不会转化。 然而，据报道，几种调节蛋白与 MYC 在 S62 和 T58 位点缺乏磷酸化有关，并且这种形式的 MYC 在 MYC 转录输出和体内致癌功能中的作用研究不足。 我们生成了条件性 c-Myc 敲入小鼠，其中野生型 MYC （MYCWT） 、T58A 突变体 （MYCT58A） 或 S62A 突变体 …

035557 STOCK Igs2 tm1(CAG-Myc*T58A/luc)Wrey /J LSL-Myc T58A mice contain a Cre recombinase regulatable Myc T58A allele under control of a CAG promoter. They are useful as a model of small cell lung cancer (SCLC).

2011年2月1日 · Expression of the c-Myc oncoprotein is affected by conserved threonine 58 (T58) and serine 62 (S62) phosphorylation sites that help to regulate c-Myc protein stability, and altered ratios of T58 and S62 phosphorylation have been observed in human cancer. Here, we report the development of 3 unique c …

MYC mutations frequently disrupt the apoptotic processes during tumorigenesis. In the present study, the effects of the MYC point mutation T58A on the progression of a cellular tumor antigen p53 (p53)-/-human breast cancer cell line was analyzed, and the mechanism of p53-independent MYC-induced apoptosis was investigated. HCC1937 cells were ...

2004年3月14日 · Phosphorylation at Ser 62 stabilizes c-Myc, whereas subsequent phosphorylation at Thr 58 is required for its degradation. Here we show that Ser 62 is dephosphorylated by protein phosphatase 2A...

In this study, Freie et al. demonstrate that a single point mutation (T58A) in MYC's phosphodegron domain stabilizes MYC and deregulates the expression of its target genes, resulting in aberrant self-renewal and apoptotic resistance in hematopoietic ...