综合考虑特应性皮炎启动因子、炎症因子以及耳肿胀度的变化，本研究选取2 nmol MC903·ear -1 剂量的剂量进一步做诱导天数探索实验，结果表明2 nmol MC903·ear -1 诱导7 d后小鼠右耳耳肿胀明显，耳组织红肿，毛细血管扩张，炎性细胞浸润明显。

A commonly used mouse model of AD has been developed using the topical application of a low-calcemic analog of vitamin D3, MC903, to induce AD-like inflammatory phenotypes that closely resemble human AD. Moreover, this model shows a minimal effect on systemic calcium metabolism that is observed in the vitamin D3-induced AD model.

2020年8月31日 · To investigate the role of mast cells (MCs) and MC-specific proteases on the immunopathogenesis of AD, a vitamin D 3 -analog (MC903) was used to induce clinical AD-like symptoms in c-kit-dependent MC-deficient Wsh -/- and the MC protease-deficient mMCP-4 -/-, mMCP-6 -/-, and CPA3 -/- mouse strains.

2020年8月31日 · MC903-Treatment Significantly Increase the Clinical Score and Ear Thickness in c-Kit Dependent Mast Cell-Deficient Mice, Independently of the Mast Cell-Specific Chymase mMCP-4. Daily topical application with MC903 induced redness, as the first sign of inflammation, after 5–8 days in the Wsh mouse strain.

2017年4月6日 · Here we report that MyD88 signaling is crucial in the pathogenesis of experimental AD induced by vitamin D3 analog MC903. The clinical signs and inflammation caused by MC903 are drastically...

2024年12月26日 · Increased ear thickness, transepidermal water loss, number of scratching events, and serum IgE levels were observed in the MC903 model. Histological analysis revealed the infiltration of granulocytes and CD3-positive T cells and an …

2023年3月22日 · AD-like skin inflammation was induced by a 7-day treatment of MC903 (calcipotriol), and ribosomal protein S6 was highly phosphorylated in inflamed tissues. MC903-induced skin inflammation was ameliorated significantly in Raptor-deficient mice and exacerbated in Pten-deficient mice.

2023年3月13日 · This article describes the MC903-induced AD mouse model using the topical application of MC903 (see Basic Protocol), skin inflammation assessment by measuring the thickening of ear skin using a dial thickness gauge (see Support Protocol 1) and assesses itching frequency which is a hallmark of AD (see Support Protocol 2).

2020年4月20日 · In the current study, we conducted a comprehensive characterization of MC903 induced ear skin inflammation in the mouse. An 11 day time course revealed skin thickening starting as early as day 4 and significantly progressing up to day 10. This chronicity was mirrored by increased transepidermal water loss in the ear skin.

The following protocol describes the preparation and application of MC903 in C57BL/6 mice and how to analyze gross skin phenotype. Application of MC903 is commonly done on ear skin in part because it facilitates quantification of ear thickness as a measure of disease activity.