2018年2月1日 · The results revealed that HPRT and 18S were the most stable reference genes in the cortices of MCAO mice and that β-actin and cyclophilin were the most stable reference genes in the OGD/R-injured N2a cells; in contrast, GAPDH and Sdha were the least stable genes in the cortices of MCAO mice and the OGD/R-injured N2a cells, respectively.

To investigate the serial changes and cellular localization of GAPDH expression, and its role in ischemia/reperfusion-induced neuronal apoptosis, the authors analyzed immunohistochemically brain areas of rats subjected to middle cerebral artery occlusion (MCAO) and reperfusion.

2025年1月30日 · In the ischemia brain, levels of phosphorylated eNOS were decreased, whereas the levels of GAPDH were increased. Taken together, these data suggest that MCAO induces hyperglycemia via enhancing glycolysis in the brain. Key words: Stroke, Ischemia, middle cerebral artery occlusion, glycolysis, glycolytic enzyme, GAPDH, eNOS.

Here we report that PARP-1 interacts with a pool of glyceradehyde-3-phosphate dehydrogenase (GAPDH) that translocates into the nucleus under oxidative/nitrosative stress both in vitro and in vivo. A well conserved amino acid at the N terminus …

2020年9月1日 · In this study, adipocyte fatty acid-binding protein (A-FABP) is identified as a novel regulator of BBB disruption because it increases the expression and activity of matrix metalloproteinase-9 (MMP-9). Treatment with a selective A-FABP inhibitor alleviated cerebral ischaemic injury and improved functional outcome in mice.

GAPDH 或G3PDH是 甘油醛-3-磷酸脱氢酶 （ glyceraldehyde-3-phosphate dehydrogenase ）的英文缩写。 GAPDH是 糖酵解 反应中的一个酶，广泛分布于各种组织中的 细胞。 该酶广泛存在于众多生物体中，并且在细胞中含量丰富，占总蛋白的10%-20%。 GAPDH基因有高度保守的序列，该酶基因为管家（house keeping）基因，几乎在所有组织中都高水平表达，在同种细胞或者组织中的蛋白质表达量一般是恒定的，且不受含有的部分识别位点、佛波脂等的诱导物质的影响而保 …

2025年1月15日 · Knockdown of Cx43 alleviated MCAO/R injury through the mediation of Cx30. The gap connexins of astrocytes play a crucial role in facilitating neuronal coordination and maintaining the homeostasis of the central nervous system.

2023年2月6日 · 因此，本研究采用大脑中动脉阻塞 (MCAO)制备脑I/R小鼠模型，探讨FB对脑I/R后氧化应激的影响，并进一步研究其潜在机制，为新药研发提供新的思路和选择。 1. 材料和方法. 1.1. 实验材料. 1.1.1. 实验动物.

2018年11月6日 · Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as the internal control. Relative mRNA expression was determined using the 2-ΔΔCT method. Six genes were analyzed: Top2a, Cdk1, Ccna2, Ccnb1, Th, and Esr1. Brain tissue was sequenced from the MCAO and control groups (n=3/group), and each experiment was performed in …

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a homotetrameric enzyme involved in a key step of glycolysis, also has a role in mediating cell death under nitrosative...