Ferumoxytol-enhanced magnetic resonance imaging can be used as an effective means to monitor the inflammatory response in the acute phase of cerebral ischemia-reperfusion injury. Furthermore, it was found that activation of the inflammatory response and apoptosis in the acute stage of cerebral ischemia-reperfusion injury is consistent.

By 3 days after MCAO, Galectin-3 + cells were significantly increased in both the core and penumbra regions of the ipsilateral striatum. The signals in the core region were colocalized with Iba1 + cells, whereas the signals in the penumbra region were mainly colocalized with GFAP + reactive astrocytes (Fig. 4a).

In a transient 90-min middle cerebral artery occlusion (MCAO) model of rats, a large ischemic lesion is formed where macrophage-like cells massively accumulate, many of which express a macrophage marker, Iba1, and an oligodendrocyte progenitor cell marker, NG2 chondroitin sulfate proteoglycan (NG2); therefore, the cells were termed BINCs (Brain ...

2022年2月17日 · A Middle cerebral artery occlusion (MCAO) was induced in C57BL/6 mice for 45 min. Coronal (approx. bregma 0–1 mm) and transversal cryosections were prepared of 24 and 72...

2019年1月24日 · Low and high scale mapping of the glial activation on brain sections of mice subjected to 30 minutes middle cerebral artery occlusion (MCAO) was correlated with that of the neuronal cell death,...

2017年6月22日 · To determine whether reactive astrocytes become phagocytic under pathological conditions, we employed a transient middle cerebral artery occlusion (MCAO) mouse model 21, 22. The mice were...

Microglia is reported with two opposing activated phenotypes, namely, classic phenotype (M1) and the alternative phenotype (M2). Inhibiting M1 while stimulating M2 has been suggested as a potential...

In a transient 90-min middle cerebral artery occlusion (MCAO) model of rats, a large ischemic lesion is formed where macrophage-like cells massively accumulate, many of which express a macrophage marker, Iba1, and an oligodendrocyte progenitor cell marker, NG2 chondroitin sulfate proteoglycan (NG2); therefore, the cells were termed BINCs (B ...

Immunofluorescence stain was used to test the co-localization of iNOS, Iba-1, and GFAP in the MCAO mouse brain section. The iNOS (green) was mainly expressed in the infarct area and not...

2024年4月1日 · Our findings revealed that EE reduced the expression of Iba1 + /CD86 + M1 microglia and hindered activation of M1 induced by MCAO while upregulating the expression of Iba1 + /CD206 + M2 microglia. qRT-PCR and serum ELISA analyses indicated that EE downregulates the release of pro-inflammatory factors (IL-1β, TNF-α) and upregulates anti ...