The reduction in Nissl staining is indicative of neuronal degeneration. A greater reduction of Nissl staining was evident in the tissues from the MCAO group compared with tissues from the NSC...

Nissl histology denotes the infarct core from 2 h up to 30 d after transient as well as permanent MCAO. Absent and attenuated MAP2 staining clearly identifies the infarct core and perilesional affected regions at all investigated times, respectively.

The infarcted region can be distinguished from the adjacent tissue ∼24 hours with H&E (Hossmann, 2008). Approximately 48 hours after permanent middle cerebral artery occlusion (MCAO) in rats, neurons lose their affinity for hematoxylin and are sometimes referred to as ‘ghost neurons' (Garcia et al, 1993).

2024年12月1日 · In vitro analyses revealed that pure Mg extract aided mouse hippocampal neuronal cell (HT-22) in defending against OGD/R injury. Additionally, the protective effects of the Mg wire on behavioral abnormalities, neural injury, blood-brain barrier disruption, and cerebral blood flow reduction in MCAO rats were verified.

2023年2月8日 · Using a middle cerebral artery occlusion (MCAO) mouse model, we identified activation of pyroptosis signal, including expression of caspase-1/11, GSDMD, and interleukin-1β/18 (IL-1β/18), in...

In this study, we tested the neuroprotective effects of hydrogen saline, which is safe and easy to use clinically, in a rat model of middle cerebral artery occlusion (MCAO). Sprague-Dawley male...

2023年1月30日 · Nissl staining also showed that compared with MCAO group rats, the Nissl bodies of brain tissue after XNJ drug intervention were clear with a 3.54-fold increased times, suggesting that XNJ improved cerebral infraction, and neurological deficits in MCAO rats. Proteomics identified 101 intersected differentially expressed proteins (DEPs).

2021年12月1日 · Focal cerebral ischemia results from a sudden reduction of cerebral blood flow (CBF) in a specific brain region (Bacigaluppi et al., 2010). The middle cerebral artery occlusion (MCAO) model is a well-known rodent stroke model that produces a cerebral infarction (an area of necrotic tissue) in one hemisphere of the brain (Bacigaluppi et al., 2010).

2023年7月4日 · 目前科研工作者通过在动物上复刻这种疾病，方便研究药物的治疗效果或者发病机制更方便。 目前常见的大鼠模型是 脑缺血再灌注 （MCAO）模型，下面对这个模型的建立以及后续的机制研究方案做一些介绍，供各位科研工作者参考。

尼氏 (Nissl)染色液 (Nissl Staining Solution)是神经生物学家广泛使用的一种Nissl染色液，用于石蜡或冰冻切片神经元细胞浆中的尼氏小体 (Nissl body)染色。 Nissl染色液染色后呈深蓝色，常用于显示脑或脊髓的基本神经…