2024年12月16日 · Here, we show that three MULTIPLE C2 DOMAIN AND TRANSMEMBRANE REGION PROTEINs (MCTPs), MCTP3, MCTP4, and MCTP6, control ARF nucleocytoplasmic partitioning and determine lateral root development. MCTP3/4/6 are highly expressed in lateral roots and specifically interact with ARF7 and ARF19 to dissolve their cytoplasmic condensates.

2019年7月9日 · MCTP3 and MCTP4 act at plasmodesmata, and are important for plant growth and development. MCTP3 and MCTP4 impact on plasmodesmata function and molecular organisation. Intercellular communication is essential for the establishment of multicellularity, and evolution gave rise to distinct mechanisms to facilitate this process.

2025年2月20日 · We further confirmed interaction between MCTP3 and MCTP4 at plasmodesmata by Förster resonance energy transfer-Fluorescence lifetime imaging (FRET-FLIM) analysis using functional pMCTP4::TagRFP-MCTP4 co-expressed with pMCTP3::eYFP-MCTP3 in the complemented mctp3-1/mctp4-1/mctp6-1 triple mutant (Figure S1H).

2024年10月31日 · The ER-PM protein tethers MCTP3, MCTP4, and MCTP6 further stabilize nascent plasmodesmata during fenestrae contraction. Genetic deletion in Arabidopsis reduces plasmodesmata formation. Our findings reveal how plants undergo incomplete division to promote intercellular communication.

2024年10月25日 · MCTP3/4/6 are highly expressed in lateral roots and specifically interact with ARF7 and ARF19 to dissolve their cytoplasmic condensates. This promotes ARF nuclear localization in lateral root...

2017年12月19日 · Recent findings have suggested that a group of highly conserved multiple C2 domain and transmembrane region proteins (MCTPs), each of which contains three to four C2 domains at the N terminus and one to four transmembrane regions at the C terminus, could function as important signaling molecules that mediate trafficking of other regulators in pl...

2018年9月23日 · We report that MCTPs are core plasmodesmata proteins that insert into the ER via their transmembrane region whilst their C2 domains dock to the PM through interaction with anionic phospholipids. A mctp3/4 loss-of-function mutant induces plant developmental defects while MCTP4 expression in a yeast Δtether mutant partially restores ER-PM tethering.

2025年2月20日 · Combining high-resolution microscopy, molecular dynamics, and pharmacological and genetic approaches, we show that cell-cell trafficking is modulated through the combined action of multiple C2 domains transmembrane domain proteins (MCTPs) 3, 4, and 6 ER-PM tethers and phosphatidylinositol-4-phosphate (PI4P) lipid.

2024年10月17日 · Xuan et al. report that MCTP3, MCTP4, and MCTP6 control ARF7 and ARF19 condensate dynamics and their nucleocytoplasmic partitioning. This promotes ARF localization to the nucleus and enhances auxin signaling, thereby facilitating lateral root formation and underscoring the role of MCTPs in shaping root architecture.

在这里，我们发现多个 C2 结构域和跨膜区蛋白 (MCTP) 家族是植物中细胞间信号转导的关键调节因子，特别在胞间连丝中充当 ER-PM 系链。 我们报告说，MCTP 是胞间连丝蛋白，通过跨膜区域插入 ER，而其 C2 结构域通过与阴离子磷脂相互作用与 PM 对接。 Atmctp3/Atmctp4 功能丧失突变体会诱导植物发育缺陷、胞间连丝功能和组成受损，而酵母 Δtether 突变体中的 MCTP4 表达部分恢复 ER-PM 束缚。 我们的数据表明，MCTP 是控制 ER-PM 接触和细胞间信号传导的独特 …