2018年7月27日 · Here we demonstrate that the SE-enriched transcriptional coactivators BRD4 and MED1 form nuclear puncta at SEs that exhibit properties of liquid-like condensates and are disrupted by chemicals that perturb condensates.

Here we demonstrate that the SE-enriched transcriptional coactivators BRD4 and MED1 form nuclear puncta at SEs that exhibit properties of liquid-like condensates and are disrupted by …

2022年2月11日 · LLPS is predicted to underlie super-enhancer function 39 (Fig. 2a) and, indeed, purified recombinant BRD4 and MED1, a core mediator subunit, were shown to phase-separate via their IDRs in vitro...

2020年3月23日 · Here we demonstrate that TAZ forms nuclear condensates through liquid–liquid phase separation to compartmentalize its DNA-binding cofactor TEAD4, coactivators BRD4 and MED1, and the transcription...

2020年3月16日 · Furthermore, the structurally disordered C terminus of BRD4L can incorporate MED1 and transcription factors into phase-separated condensates at enhancer sites in chromatin 18, 19; the...

2023年9月21日 · Shrinivas et al. have analyzed how the sequence of the enhancer affected the LLPS of MED1 and OCT4 mixture. DNA originals with specific binding sites facilitate the condensation formation of MED1 and OCT4 mixture in low protein concentrations.

Purified recombinant MED1-IDR-GFP fusion protein exhibited concentration-dependent liquid-liquid phase separation. Droplets of MED1-IDR could incorporate and concentrate purified OCT4-GFP to form heterotypic droplets (PMID:30449618). Literature supporting the LLPS: 29930091, 29930094, 30449618

2024年3月18日 · Furthermore, in vitro assays demonstrate distinct physio-chemical properties of liquid-liquid phase separation (LLPS) for 6KR versus 6KQ MED1 IDRs, and for non-acetylated versus CBP-acetylated WT MED1 IDR fragments.

Studies have shown that MED1 LCD and Mediator holocomplex undergo LLPS in vitro. Substitution of serine residues in MED1 with alanine abolishes phase separation, indicating the importance of serine-mediated polar intermolecular interactions in LLPS.

We achieve this advance by driving optogenetic TFs to undergo liquid-liquid phase separation (LLPS) and to form TF droplets around their target promoters. Eukaryotic cells are highly compartmentalized into specific organelles, which are either membrane enclosed or …