Learn how spatially resolved transcriptomics with MERFISH technology enables the deep profiling of the expression of RNAs in single cells in their native context revealing cell type, state, organization, interactions, and function within cell culture and tissue.

2025年2月21日 · MERFISH is an imaging method capable of simultaneously measuring the copy number and spatial distribution of hundreds to thousands of RNA species in single cells. This technique was introduced in the paper:

2015年4月24日 · Numerous RNA species can be identified, counted, and localized in a single cell by using MERFISH, a single-molecule imaging approach that uses combinatorial labeling and sequential imaging with encoding schemes capable of detection and/or correction of errors.

Here we report MERFISH, a highly multiplexed smFISH imaging method that substantially increases the number of RNA species that can be simultaneously imaged in single cells by using combinatorial labeling and sequential imaging with error-robust encoding schemes.

2015年4月24日 · Here, we report multiplexed error-robust fluorescence in situ hybridization (MERFISH), a single-molecule imaging approach that allows the copy numbers and spatial localizations of thousands of RNA species to be determined in single cells.

2023年12月13日 · By imaging approximately 10 million cells with MERFISH and integrating the MERFISH data with a whole-brain scRNA-seq dataset, we determined the spatial organization of more than 5,000 ...

MERFISH combines the power of single-cell transcriptomics with spatial biology by directly visualizing and counting up to a billion RNA transcripts from 100’s to 10,000 genes across 1cm2 tissue slices with single-cell resolution.

2019年5月22日 · Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single...

2018年3月19日 · As an image-based single-cell transcriptomics approach, multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows hundreds to thousands of RNA species to be identified, counted...

2022年10月21日 · Using this epigenomic MERFISH method, we demonstrated the ability to profile epigenetic modifications on chromatin, including modifications marking active promoters, putative enhancers, and silent chromatin, in individual cells with high spatial and genomic resolution, as well as high genomic throughput.