The first enzyme of the PET degradation pathway, PETase, cleaves this plastic into the intermediates MHET (Mono-(2-hydroxyethyl)terephthalic acid) and minor amounts BHET (Bis-(2-hydroxyethyl)terephthalic acid). MHETase hydrolyses the ester bond of MHET forming terephthalic acid and ethylene glycol. Enzymatic PET degradation by PETase and MHETase

2019年4月12日 · Two α/β-hydrolase fold enzymes (α/β-hydrolases), PETase and MHETase, work together to degrade PET in two steps via MHET, yielding TPA and ethylene glycol—the building blocks required for a ...

2023年11月9日 · A study on the computational design of enzymes for plastic (MHET and PET) degradation yielded an efficient dual enzyme degradation system for PET recycling with a substantially increased...

Ideonella sakaiensiscells bind to the PET surface and degrade it into mono(2-hydroxyethyl) terephthalic acid (MHET), a heterodimer of terephthalic acid (TPA) and ethylene glycol, using a secreted PET hydrolase, or PETase. The first PETase was discovered in I. sakaiensis, and it works by hydrolyzing the ester bonds found in PET with high ...

2021年8月6日 · In 2016, one of the two enzymes involved in the polyethylene terephthalate (PET) degradation pathway of Ideonella sakaiensis 201-F6, MHETase, was found to exhibit a strong ability to degrade the PET monomer mono-(2-hydroxyethyl)terephthalate (MHET) at room temperature, converting it back into the precursors used in PET production. MHETase ...

2018年1月26日 · Based on structural and site-directed mutagenesis experiments, the detailed process of PET degradation into MHET, terephthalic acid, and ethylene glycol is suggested.

PET的酶水解产生对苯二甲酸 (TPA)、乙二醇 (EG)、对苯二甲酸单 (2-羟乙基)酯 (MHET)和对苯二甲酸双 (2-羟乙基)酯 (BHET)的混合物作为主要产品。 这项工作开发了一种新的方法来量化生物催化剂的水解活性，使用 BHET 作为模型底物。 该协议可用于筛选用于 PET 解聚反应的酶，以及其他应用。 非常好的拟合（R 2 = 0.993) 实验数据和数学模型之间证实了 Michaelis-Menten 方程分析 BHET 浓度 (8-200 mmol L -1 ) 对特异腐质霉 角质酶 (HiC)催化的初始水解速率影响的可行 …

最近开发的用于聚对苯二甲酸乙二醇酯 (PET) 解聚的酶，例如 FAST-PETase 和 LCC-ICCG，会受到 PET 中间产物对苯二甲酸单 (2-羟乙基)酯 (MHET) 的抑制。 因此，PET 酶促转化为其组成单体对苯二甲酸 (TPA) 和乙二醇 (EG) 的效率很低。 在这项研究中，从结构数据库中选择了与嗜热羧酸酯酶（Est30）相对应的蛋白质支架（1TQH），并在计算机中重新设计。 在设计中，双变体 KL-MHETase (I171K/G130L) 的蛋白质熔解温度 (67.58 °C) 与 PET 水解酶 FAST-PETase (67.80 …

5 天之前 · The breakdown of PET into MHET follows a sequence of hydrolytic reactions that dismantle the polymer’s ester linkages. This process is primarily driven by enzymatic hydrolysis, where PET-degrading enzymes like PETase cleave ester bonds, yielding soluble intermediates. MHET serves as a transitional product, bridging PET degradation to its ...

2024年9月27日 · TmFae-PETase能降解PET塑料并其转化为MHET和TPA等产物（图3b）。 为评估TmFae-PETase的PET水解能力，将其与已报道的PET水解酶进行功能比较，从系统发育树的每个分支上选择一个代表性的PET水解酶，分别是IsPETase、FsC和LCC。