Basal levels of microglia were quantified in the hippocampi of Cx3Cr1 GFP /+ mice at P0, P5, P10, P15, P20, P25, P30, P40, and P60. Seizure susceptibility and seizure‐induced microglial activation were assessed in response to febrile seizures (lipopolysaccharide followed by hyperthermia) and kainic acid‐induced status epilepticus.

2023年11月24日 · Depletion of microglia from P5 leads to an accumulation of PSVue labeling, indicating that microglia indeed participate in synaptic pruning during the early postnatal period in the hypothalamus...

For whisker plucking experiments, all whiskers were plucked unilaterally, either during the critical period for plasticity (P0-P5, 'early plucking') or during the late period when vascular...

2023年8月16日 · At P0, perivascular astrocytes exhibited simple main branches aligned along the scaffold of blood vessels (Fig. 1a), and astrocyte cell bodies were in close apposition with microvessels compared...

Embryonic (E19; n = 7) and postnatal (P0;n = 14; P5; n = 17) mice were also injected with crystals of biocytin in the entorhinal cortex or hippocampus. After ether anesthesia, mice were placed onto a stereotaxic frame mounted on chilled ice.

2023年2月18日 · Together our results reveal early deficits in the hippocampal neurogenic niche of the 3xTg mice, with a decrease in NSCs as early as P5, and a reduction in neurogenesis detected at 1 month of...

For mice neuronal cultures, starting from E15.5 each day that passes will render cultures with higher glial contamination that's why normally they are used at E16-E17, specially for cortical...

P0, the hippocampus was nearly devoid of microglia (Fig. 1). While a slightly increased number of cells was found within the hippocampus at P5, a dramatic upsurge of microglia occurred by P10...

2005年3月31日 · In the present work, we investigated the postnatal development (P0, P5, P10, P15, and P21) of this system (AgRP transcript and peptide) in the mouse brain using in situ hybridization and immunohistochemistry. At all stages, AgRP mRNA expression was detected exclusively in the Arc.

2016年9月1日 · In WT mice the density of apoptotic neurons progressively decreased from birth to P15 with a statistically significant drop from P10 onward (Student's t-test: P0/P10 p = 0.041; P0/P15 p = 0.021) whereas reduction in the density of apoptotic neurons was not statistically significant across five-day intervals. These data demonstrate that, in ...