2019年9月2日 · The bipolar M1/M2 paradigm which describes the polarization of macrophages in cell culture with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) (M1) or interleukin-4 (IL-4) and IL-13 (M2)...

2023年12月7日 · Using single-cell RNA sequencing, we found that APG-2575 polarized M2-like immunosuppressive macrophages toward the M1-like immunostimulatory phenotype with increased CCL5 and CXCL10 secretion,...

In this review, we discuss emerging evidence highlighting the redefinition of TAM subpopulations and functions in the TME and the possibility of separating macrophage subsets with distinct functions into antitumor M1-like and pro-tumor M2-like TAMs during the development of tumors.

2023年8月16日 · 相比之下，m1和m2巨噬细胞标记物的tam表达与患者的临床预后无关，这些标记物被广泛用于定义具有抗肿瘤和原肿瘤功能的巨噬细胞。 这些发现支持一种观点，即M1和M2表型可能定义了体外细胞的极端状态，但不一定反映了体内TAM的复杂性。

In summary, M1 and M2 macrophages are functionally distinct and key modulators in host immune system against tumors. In contrast to M1, M2 mechanisms incorporate location-related, mutually connected, and cascade-like reactions.

In this study, we innovatively applied mIHC to femoral head specimens (hard tissues) and found that as NONFH progression from progressive stage to end-stage, macrophage infiltration in the reparative area as well as macrophage M1/M2 imbalance increased, while the number of blood vessels declined.

2024年11月4日 · Our investigation, involving a comprehensive analysis of single-cell data from liver cancer and mIHC experiments, revealed that GSDME is expressed predominantly in M2-like macrophages.

2024年12月25日 · Inhibiting M2 macrophage function or promoting their conversion into anti-tumor M1 macrophages is a potential therapeutic strategy. The interplay between C1q and high mobility group box 1 (HMGB1) is vital to immune response modulation.

2024年9月1日 · One of the mechanisms underlying their protective effects against RA is the prevention of M2 macrophage ferroptosis, which can rebalance the M1/M2 ratio, suppress osteoclasts-promoting cytokines secreted from M1 macrophages and maintain a normal immunological microenvironment.

2021年6月4日 · We examined the infiltration of immune cells and expression of PD-L1 in immune cells using fluorescent multiplex immunohistochemistry (mIHC) stained with CD8/CD68/CD163/PD-L1 antibodies.