2018年7月24日 · Interestingly, mIHC detected significantly higher Treg numbers than FACS and showed preferential CD4 + T cell distribution in the tumor stroma.

2024年10月23日 · Using a supervised representation learning algorithm, we identified a Ki67 + proliferating regulatory T cells (Tregs) population expressing high levels of activated and immunosuppressive...

2024年5月7日 · We employed multicolor fluorescence immunohistochemistry (mIHC) to stain Foxp3, cytokeratin, and nuclei on an HCC tissue microarray (TMA). Leveraging liver cancer transcriptome data from TCGA, we built a prognostic model focused on Treg-associated gene sets and represented it with a nomogram.

The aim is to investigate the impacts of using multiplex immunochemistry (mIHC) staining to analyses the co-expression of programmed death ligand-1 (PD-L1) and tumor infiltrating lymphocytes (TILs) [CD8 + T cells and Forkhead Box Protein 3 (FOXP3) + regulatory T cells (Tregs)] in different oral diseases, and oral squamous cell carcinoma (OSCC).

Methods: We employed multicolor fluorescence immunohistochemistry (mIHC) to stain Foxp3, cytokeratin, and nuclei on an HCC tissue microarray (TMA). Leveraging liver cancer transcriptome data from TCGA, we built a prognostic model focused on Treg-associated gene sets and represented it with a nomogram.

Multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) technologies, which allow the simultaneous detection of multiple markers on a single tissue section, have been introduced and adopted in both research and clinical settings in response to …

2020年12月8日 · Purpose: The aim is to investigate the impacts of using multiplex immunochemistry (mIHC) staining to analyses the co-expression of programmed death ligand-1 (PD-L1) and tumor infiltrating lymphocytes (TILs) [CD8 + T cells and Forkhead Box Protein 3 (FOXP3) + regulatory T cells (Tregs)] in different oral diseases, and oral squamous cell ...

2024年11月5日 · 为了验证Ki67+ Tregs的预测价值，我们在独立队列中进行了多重免疫组化(mIHC)分析。 结果显示，Ki67+ Tregs在肿瘤中的丰度可以有效区分对免疫治疗有完全反应和无完全反应的患者(图6a, b)。

22 小时之前 · Tregs (yellow) were identified using colocalization of CD4 and FOXP3. Scale bar, left, 20 μm; right, 5 μm. B Intensity of Treg (yellow) and IL1R2 (violet) was calculated using ImageJ according to the line on picture, related to Fig. 2A. C Representative mIHC staining of IL1R2+Treg in TMA (n = 69) with different TNM stages. Scale bar, left, 20 ...

2021年2月17日 · The TSA Opal multiplex immunohistochemistry (mIHC) protocol (PerkinElmer) has been used to characterize immune infiltration in human cancers. This technique allows multiple biomarkers to be simultaneously stained in a single tissue section, which helps to elucidate the spatial relationship among ind …