Additional proteins, called minor pilins, are present in lower abundance, but are essential for the assembly of the pilus or for its specific functions. One class of minor pilins is required to initiate the formation of pili, and may form a complex similar to that identified in the related type II secretion system.

2020年10月7日 · Here, using genetics, cell biology, proteomics and cryo-electron tomography, we demonstrate that the PilY1 protein and four minor pilins, which are widely conserved in T4aP systems, are essential...

2021年6月11日 · In this study, we show that the minor pilin PilA5 is essential for natural transformation but is dispensable for motility and flocculation. In contrast, a set of minor pilins encoded by the pilA9 - slr2019 transcriptional unit are necessary for motility but are dispensable for natural transformation.

2010年5月7日 · Using specific antibodies for each protein, we showed that the minor pilins FimU, PilV, PilW, PilX, and PilE were processed by the pre-pilin peptidase PilD and incorporated throughout the growing pilus filament.

Here, we investigated the role of the P. aeruginosa minor pilin PilE in pilus assembly. We show that PilE interacts with the major pilin subunit and other core minor pilins, supporting its proposed role as a connector of a minor pilin subcomplex to the major pilin-containing fiber during initiation of pilus assembly.

2015年1月2日 · Here we show that Pseudomonas aeruginosa minor pilins prime pilus assembly and traffic the pilus-associated adhesin and anti-retraction protein, PilY1, to the cell surface. PilV, PilW, and PilX require PilY1 for inclusion in surface pili …

2018年5月18日 · Type IV pili are expressed by a wide range of prokaryotes, including the opportunistic pathogen Pseudomonas aeruginosa. These flexible fibres mediate twitching motility, biofilm maturation, surface adhesion, and virulence. The pilus is composed mainly of major pilin subunits while the low abundance …

2025年2月12日 · This minor pilin-PilY1 complex is ultimately incorporated into the tip of the growing pilus fiber after successful nucleation of pilus assembly (41, 42). Thus, CpaL, together with the putative Tad minor pilins CpaJ and CpaK, could similarly form a complex that is incorporated at the tip of the pilus fiber in C. crescentus.

2020年11月29日 · Additional proteins, called minor pilins, are present in lower abundance, but are essential for the assembly of the pilus or for its specific functions. One class of minor pilins is required to initiate the formation of pili, and may form a complex similar to that identified in the related type II secretion system.

2019年4月15日 · Many bacteria possess filamentous appendages called pili. These filaments, which include the type IV pili (T4P), chaperone-usher pili 1 and Gram-positive pili 2, are polymers of thousands of copies...