细胞名称：MIO-M1 视网膜Muller干细胞 组织来源： 视网膜 培养条件：DMEM+10% FBS 形 态：贴壁 细胞培养步骤 一．培养基及培养冻存条件准备： 1） 准备培养基；北美胎牛血 …

MIO-M1人视网膜Muller干细胞. 产品编号：YS1695C; 产品类别：人源细胞系; 生长特性：贴壁生长; 培养体系：DMEM（高糖）+10%FBS; 传代方法 ：1：2传代; 细胞形态：上皮细胞样; 冻存条件：无血清细胞冻存液

MIO-M1 cells and similar Muller glial cell lines derived from adult human retina exhibit neural stem cell characteristics. Stem Cells 25:2033-2043(2007) PubMed=22065927; PMCID=PMC3209432 Hollborn M., Ulbricht E., Rillich K., Dukic-Stefanovic S., Wurm A., Wagner L., Reichenbach A., Wiedemann P., Limb G.A., Bringmann A., Kohen L.

人muller细胞MIO-M1取自女性供体。该细胞为自发永生化细胞系。

We first reported the Müller glial characteristics of the spontaneously immortalized human cell line MIO-M1, but recently we have derived similar cell lines from the neural retina of several adult eye donors. Since immortalization is one of the main properties of stem cells, we investigated whether these cells expressed stem cell markers.

A spontaneously immortalized Müller cell line was characterized that retains the characteristics of primary isolated cells in culture. To the authors' knowledge, it constitutes the first human Müller cell line reported in the literature. It has been named MIO-M1 (Moorfields/Institute of Ophthalmolog …

我们首先报道了自发永生化人细胞系 MIO-M1 的 Müller 神经胶质特征，但最近我们从几个成年眼供体的神经视网膜中获得了类似的细胞系。 由于永生化是干细胞的主要特性之一，我们研究了这些细胞是否表达干细胞标志物。

Karyotypic analysis of MIO-M1 cells confirmed that these cells were of human derivation, displaying two X chromosomes, in accordance with the female origin of the cells. They display structural abnormalities observed in cells in long-term culture, with a near triploid modal chromosome number (71 ± 5) and a satellite chromosome.

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