miRNA介导的RISC简称为 miRISC ( miRNA-containing RNA induced silencing coplex)，也被称作miRNA核糖核蛋白复合体 ( miRNP)。 miRISC复合体除了包括成熟 miRNA外，还包含Dicer蛋白和多种其他相关蛋白。 其与 RISC结合的原理与siRNA类似，通过miRNA: miRNA*双体两端热力学稳定性的分析，可以分为两类结合： 优势结合与等势结合。 以 dsRNA为例，当双链中两根支链的稳定性相似或相同时，它们结合进入RISC的概率也相似或相同，因此称为等势结合（如图一所 …

2012年6月5日 · This Review describes recent advances, with an emphasis on how the miRNA-mediated silencing complex (miRISC) controls gene expression by inhibiting translation and/or mRNA decay, and how trans ...

2023年3月24日 · miRNA 是一种内源性的微小非编码 RNA，长度一般在 21-23 个核苷酸之间，具有在翻译水平调控基因表达的功能[2]。 miRNA 的发生 “成长” 需要几个步骤：最初，miRNA 基因由 RNA 聚合酶 Ⅱ 转录，生成较长的前体 pri-miRNA，而后形成发夹状的 pre-miRNA [3,4]。 最后在细胞质中被切割形成一个成熟的 miRNA 双链。 至此，miRNA 从 >1000 个 nt 的 “大可爱” 摇身一变成为了仅约 22 个 nt 的 “小精灵”。

MicroRNA (miRNA)-induced silencing complexes (miRISCs) repress translation and promote degradation of miRNA targets. Target degradation occurs through the 5'-to-3' messenger RNA (mRNA) decay pathway, wherein, after shortening of the mRNA poly (A) tail, the removal of the 5' cap structure by decapping triggers irreversible decay of the mRNA body.

miRISC is a multi-protein assembly that uses microRNAs (miRNAs) to identify mRNAs targeted for repression. Dozens of miRISC-associated proteins have been identified, and interactions between many factors have been examined in detail.

The miRISC functions to identify the target transcripts first and then silence the gene expression of the target mRNA. The miRISC uses imperfect base complementarity to identify the target sequence in the mRNA. The target sites for the miRNA …

2018年5月3日 · miRISC is a multi-protein assembly that uses microRNAs (miRNAs) to identify mRNAs targeted for repression. Dozens of miRISC-associated proteins have been identified, and interactions between many factors have been examined in detail.

2012年6月5日 · In this review we describe recent discoveries, with an emphasis on how miRISC post-transcriptionally controls gene expression by inhibiting translation and/or initiating mRNA decay, and how trans-acting factors control miRNA action.

2013年7月17日 · MicroRNA (miRNA)-induced silencing complexes (miRISCs) repress translation and promote degradation of miRNA targets. Target degradation occurs through the 5′-to-3′ messenger RNA (mRNA) decay pathway, wherein, after shortening of the mRNA poly (A) tail, the removal of the 5′ cap structure by decapping triggers irreversible decay of the mRNA body.

2022年2月13日 · The miRISC functions to identify the target transcripts first and then silence the gene expression of the target mRNA. The miRISC uses imperfect base complementarity to identify the target sequence in the mRNA. The target sites for the miRNA are usually located in the 3′UTR of the mRNA.