Micro ribonucleic acid (microRNA, miRNA, μRNA) are small, single-stranded, non-coding RNA molecules containing 21–23 nucleotides. [1] .

This knowledge base provides an introduction to miRNA (microRNA) biogenesis, including the role of Drosha and Dicer enzymes and the RISC complex in the canonical pathway. You'll learn how miRNAs bind to mRNA or 3'UTRs to knockdown genes and become familiar with the miRNA nomenclature system.

2024年10月18日 · Structure: Mature miRNAs are single-stranded RNA molecules. In the cytoplasm, Dicer cuts the pre-miRNA into a mature miRNA duplex approximately 22 nucleotides in length. There are two strands to this: the guide strand (the active miRNA) and the passenger strand, or miRNA.

A short, 70-nucleotide stem-loop structure known as pre-miRNA is formed from primary miRNA in the cell nucleus. This processing is performed in animals by a protein complex known as the Microprocessor complex, consisting of the nuclease Drosha …

2020年3月17日 · Many specific features of miRNA structure, their encoding in the genome and ways of expression and maturation have been elucidated; the main functions of miRNAs in the cell and their protein partners have been determined.

2019年10月7日 · Structurally, the siRNA is a 21-23 nucleotide long RNA duplex having a dinucleotide 3’ overhang. Whereas the miRNA is made up of 19-25 nucleotide RNA hairpins which form duplex by binding with each other. Pictorial representation of the structural difference between siRNA and miRNA.

2014年11月25日 · Using specific nucleases, T1, V1 and S1 as well as NMR, UV/Vis and CD spectroscopies, we found that miR-21, miR-93 and miR-296 can adopt hairpin and/or homoduplex structures. The secondary structure of those miRNAs in solution is a function of RNA concentration and ionic conditions.

To address the gap of structure availability, miRVim has been developed, providing a repository of human miRNA structures derived from both two-dimensional (MXFold2, CentroidFold, and RNAFold) and three-dimensional (RNAComposer and 3dRNA) structure prediction algorithms.

2024年4月2日 · The microRNA (miRNA) are small RNA molecules with a remarkably intricate structure that belies their substantial regulatory impact on gene expression. The structure of miRNAs is a key factor in understanding their function and versatility in orchestrating various cellular processes.

2024年5月30日 · Mature microRNAs (miRNAs) are a family of naturally occurring, 21–25 nucleotide-long non-coding RNA molecules. MicroRNAs are partly complementary to one or more messenger RNA (mRNA) molecules, and their primary purpose is to inhibit gene expression via translational repression, mRNA cleavage, and deadenylation.