Nuclear RIPK3 and MLKL contribute to cytosolic necrosome
2018年1月22日 · We report that RIPK3 and MLKL continuously shuttle between the nucleus and the cytoplasm, whereas RIPK1 is constitutively present in both compartments. During TNF …
MLKL Compromises Plasma Membrane Integrity by Binding to ...
2014年5月22日 · We fused full-length MLKL, N-terminal 4HBD–BR, and C-terminal KLD to GFP and analyzed the subcellular localization of the different fusion proteins by confocal microscopy.
Enhanced channel activity by PI(4,5)P2 ignites MLKL-related
2022年10月18日 · The physical interaction between MLKL and phosphatidylinositol phosphates (PIPs), including PI(4,5)P 2, facilitates MLKL-mediated liposome leakage and the necrotic …
Mixed lineage kinase domain-like is a key receptor interacting …
2012年3月15日 · In the current study, we report the identification of mixed lineage kinase domain-like (MLKL) as a key RIP3 downstream component of TNF-induced necrosis. Through …
MLKL polymerization-induced lysosomal membrane …
2023年11月23日 · We have determined that activated MLKL translocates to the lysosomal membrane during necroptosis induction. The subsequent polymerization of MLKL induces …
Plasma membrane translocation of trimerized MLKL protein is …
The mixed lineage kinase domain-like protein (MLKL) has recently been identified as a key RIP3 (receptor interacting protein 3) downstream component of tumour necrosis factor (TNF) …
Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down …
2018年11月1日 · We found that MLKL protein expression was drastically elevated in Schwann cells in the injured nerves and underwent a novel RIP3-independent phosphorylation event …
MLKL polymers are proteinase K-resistant, high molecular weight ...
MLKL polymers are proteinase K-resistant, high molecular weight complexes. (A) Establishment of the HeLa:GFP-RIPK3:MLKL cell line. Endogenous MLKL was knocked out with CRISPR …
Site-specific ubiquitination of MLKL targets it to endosomes and ...
2022年1月9日 · We report that conjugation of K63-linked polyubiquitin chains to distinct lysine residues in the N-terminal HeLo domain of phosphorylated MLKL (facilitated by the ubiquitin …
ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell …
2017年4月6日 · Here, we show that, during necroptosis, MLKL-dependent calcium (Ca 2+) influx and phosphatidylserine (PS) exposure on the outer leaflet of the plasma membrane preceded …