2022年1月3日 · The MLL-PTD was examined by real-time quantitative polymerase chain reaction (RQ-PCR) at the diagnosis, before transplantation and the fixed time points after transplantation. Detectable MLL-PTD/ABL > 0.08% was defined as MLL-PTD positive in this study. The 48 patients included 33 AML patients and 15 MDS-EB patients.

急性白血病可发生MLL基因重排，其中MLL基因部分串联重复 (MLL-PTD)为MLL基因重排中最为常见的形式之一，主要存在于核型正常及具有第11号染色体三体的急性髓细胞白血病 (AML)中。 作者拟就MLL基因结构、功能、MLL-PTD在AML发生、发展中的作用机制，及其可作为AML潜在治疗靶点等方面进行综述。 引用本文: 卞梅茹, 陈伟, 吴庆运, 等. 混合谱系白血病基因部分串联重复在急性髓细胞白血病中的研究进展 [J] . 国际输血及血液学杂志, 2015, 38 (3) : 236-239. DOI: …

Partial tandem duplication (PTD) of the KMT2A (MLL) gene is detected in approximately 5-10% of cases of acute myeloid leukemia (AML) 1–5 and within cases showing normal karyotype, confers a worse prognosis. 2,6,7 In these patients, the duplications are variable in size and most commonly involve exons 2 or 3, spanning through exon 6 or exons 8 ...

In this review, we will discuss the normal biological roles of MLL1 and its fusion partners, how these roles are hypothesized to be dysregulated in the context of MLL1 rearrangements, and the clinical manifestations of this group of leukemias.

2022年1月1日 · Using a combination of cell biology and proteomic approaches in leukemic cell lines and patient-derived xenograft models (PDX) of AML, we made the surprising discovery that in leukemic cells, protein levels of MLL-PTD are dramatically reduced despite MLL-PTD transcripts being readily detectable.

2016年6月8日 · This study provides important insights for understanding the relative importance of different mutations for defining a targeted therapeutic strategy for MLL-PTD AML patients.

2003年5月15日 · We performed reverse-transcriptase polymerase chain reaction (RT-PCR) analysis to detect expression of MLLwt and MLL PTD (Figures 1 and 2). MLLwt was shown to be widely expressed in acute...

2022年1月3日 · The MLL-PTD was examined by real-time quantitative polymerase chain reaction (RQ-PCR) at the diagnosis, before transplantation and the fixed time points after transplantation. Detectable MLL-PTD/ABL > 0.08% was defined as MLL-PTD positive in this study. Results: The 48 patients included 33 AML patients and 15 MDS-EB patients. The median follow ...

2018年11月29日 · In the current study, we investigated the mechanisms for HIF1A activation by Mll -PTD. In normoxia, HIF1A is hydroxylated by prolyl hydroxylases (PHD), resulting in rapid protein degradation via ubiquitination.

2019年5月26日 · In this study, we developed an MLL-PTD detection assay that uses long-range PCR on extracted DNA, a commonly processed sample type for AML molecular testing, to reduce sample processing requirements. Methods: DNA was extracted from de-identified 72 whole blood and 30 bone marrow aspirate specimens.