MLKL是TNF（肿瘤坏死因子）诱导的坏死性凋亡过程中的关键靶标，它作为RIPK1和RIPK3下游的效应蛋白发挥作用。 RIPK3在Ser227位点被磷酸化，此磷酸化为MLKL激活所必需。 MLKL的激活导致MLKL从细胞质到不同细胞膜的构象开关和易位，在质膜上使得膜丧失完整性，并最终导致坏死性凋亡。 MLKL与RIPK3的相互作用具有物种特异性，人类MLKL仅与人类RIPK3相互作用。 03. 各种胞外或胞内信号直接或通过RIPK1激活RIPK3，RIPK3和MLKL是坏死性凋亡的重要组成 …

2021年5月5日 · Mixed lineage kinase domain-like protein (MLKL) emerged as executioner of necroptosis, a RIPK3-dependent form of regulated necrosis. Cell death evasion is one of the...

2021年11月2日 · Mass spectrometric analysis identified a single phosphorylated MLKL peptide FSNRpSNICR with Ser83 phosphorylation (Fig. 1b). We then introduced single site mutation to test how it affects...

2017年4月6日 · Here, we show that, during necroptosis, MLKL-dependent calcium (Ca 2+) influx and phosphatidylserine (PS) exposure on the outer leaflet of the plasma membrane preceded loss of PM integrity. Activation of MLKL results in the generation of broken, PM “bubbles” with exposed PS that are released from the surface of the otherwise intact cell.

2024年12月25日 · MLKL (Mixed Lineage Kinase Domain Like Pseudokinase) is a Protein Coding gene. Diseases associated with MLKL include Maturity-Onset Diabetes Of The Young and Caspase 8 Deficiency. Among its related pathways are RIPK1-mediated regulated necrosis and Ion channel transport.

Mixed lineage kinase domain-like pseudokinase (MLKL) is considered to be the key mediator of necroptosis. After necroptosis initiation, MLKL together with RIP1 and RIP3 forms necrosome, and the phosphorylated MLKL homo-oligomerizes and translocates to the plasma membrane (PM) to induce cell death.

2016年2月12日 · The pseudokinase, MLKL (mixed-lineage kinase domain-like), is the most terminal obligatory component of the necroptosis cell death pathway known. Phosphorylation of the...

2017年4月6日 · Here, we show that, during necroptosis, MLKL-dependent calcium (Ca 2+) influx and phosphatidylserine (PS) exposure on the outer leaflet of the plasma membrane preceded loss of PM integrity. Activation of MLKL results in the generation of broken, PM “bubbles” with exposed PS that are released from the surface of the otherwise intact cell.

2017年7月18日 · Activation of the pseudokinase mixed lineage kinase domain-like (MLKL) upon its phosphorylation by the protein kinase RIPK3 triggers necroptosis, a form of programmed cell death in which rupture of cellular membranes yields release of intracellular components.

2013年6月7日 · Receptor interacting protein 3 (RIP3) is a protein kinase essential for TNF-induced necroptosis. Phosphorylation on Ser-227 in human RIP3 (hRIP3) is required for its interaction with human mixed lineage kinase domain-like (MLKL) in the necrosome, a signaling complex induced by TNF stimulation.