The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechanism of the inhibition has not been elucidated yet.

2024年11月15日 · 病毒的包膜蛋白决定了病毒侵染的宿主范围，目前，根据逆转录病毒基因转移系统的亲嗜性，可分为4种：① 单嗜性 (Ecotropic)，包膜蛋白为env (gp70)，只能感染小鼠和少数品种的大鼠细胞；② 双嗜性 (Dualtropic)，包膜蛋白采用鼠白血病病毒的包膜蛋白4070A，能同时感染小鼠和人的细胞；③ 多嗜性 (Amphotropic)，包膜蛋白采用鼠白血病病毒的包膜蛋白10A1，既能感染小鼠和人的细胞，也能感染其它哺乳动物细胞；④ 泛嗜性 (Pantropic)，包膜蛋白采用水 …

The viral glycoproteins are expressed on the membrane as trimer of a precursor Env, which is cleaved into SU and TM by host furin or furin-like proprotein convertases. This cleavage is essential for the Env incorporation into virus particles.

The host range of recombinant MoMuLV vectors is dependent on the specificity of the viral envelope. The ecotropic env gene produces particles which infect only rodent cells. The amphotropic env gene allows infection of rodent and non-rodent cells, including human cells.

2012年4月30日 · The spike protein Env on the surface of the retrovirus murine leukemia virus (MLV) matures by two proteolytic cleavage events mediated by cellular furin and the viral protease (1–3). Env is composed of an 80-kDa transmembrane precursor protein in the rough endoplasmic reticulum of the infected cell (4, 5).

2010年9月30日 · The cytoplasmic tail domain (CTD) of retroviral envelope (Env) proteins has been implicated in modulating Env incorporation into viral particles. We generated a panel of murine leukemia virus (MLV) Env mutants and analyzed their ability to be recruited to human immunodeficiency virus-1 (HIV-1) assembly sites.

鼠白血病病毒（MLV或MuLV）是感染老鼠的逆转录病毒，它可以在老鼠中引起白血病，一些 MLV 可能会感染其他脊椎动物。 MLV有两种——外源性病毒和内源性病毒。 MLV 具有正义单链 RNA (ssRNA) 基因组，该基因组通过逆转录过程通过 DNA 中间体进行复制。 分类. 鼠白血病病毒是属于逆转录病毒家族丙型逆转录病毒 (Gammaretrovirus)的逆转录病毒。 复制 MLV 的病毒颗粒具有 C 型形态，由电子显微镜确定。 MLV 包括外源性和内源性病毒。 外源形式作为新的感染从一个 …

The Env protein of MLV, like that of other orthoretroviruses, is translated from a singly spliced mRNA. There is an overlap of 58 bases between the end of the Pol coding region and the beginning of the Env coding region.

The C-terminal R peptide of ecotropic murine leukemia virus (MLV) envelope protein (Env) negatively controls membrane fusion activity. The R peptide cleavage during virion maturation activates its fusogenicity and is required for viral entry. We analyzed fusogenicity and transduction efficiency of m …

M-MLV本质是一种将单链RNA、DNA或RNA-DNA杂合体（利用引物）合成互补DNA链的DNA聚合酶。 可用于 RT-PCR反应 制备 cDNA文库 或合成第一链cDNA。 和 AMV 逆转录酶 相比，M-MLV 逆转录酶缺乏DNA内切活性， RNase H活性 更低。 （注：RNase H是指核糖核酸酶H，是一种核糖核酸内切酶，可以特异性地水解DNA-RNA杂合链中的RNA。 M-MLV（H-)是在M-MLV的基础上，对蛋白序列进行突变优化，进一步降低RNaseH活性。 应用示例： Q-PCR. EZassay M …