2011年4月25日 · Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. In this chapter, brain removal and separate dissection of multiple brain regions...

2024年6月1日 · Offers a detailed, step-by-step dissection guide for different brain regions, including the hippocampus, cerebral cortex, corpus striatum, thalamus, cerebellum, and medial prefrontal cortex, from rodent brains, accompanied by high-resolution images that provide anatomical clarity.

2021年2月15日 · We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs.

Despite the importance of this issue, there is no validated method enabling dissection of the hippocampus from fresh mouse brain with precision sufficient for reliable removal of the choroid plexus. An alternative approach providing perfect precision is laser microdissection from frozen brain slices [ 8 ], but this method is very expensive and ...

Here we outline a protocol for subregion-specific dissection from the adult mouse. Place container of 1× Cutting Solution in a filled ice bucket, and transfer ~15 ml of the solution into a 20 ml beaker also on wet ice. Oxygenate the solutions in both the bottle and beaker for at least 20 min before beginning (see below).

2021年2月15日 · In this procedure, we have focused on male mouse C57Bl/6J (6-8 week old) brain removal and dissection into multiple regions using neuroanatomical landmarks to identify and sample discrete functionally-relevant and behaviorally-relevant brain regions.

2011年1月1日 · Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. In this chapter, brain removal and separate dissection of multiple brain regions from a single brain are described in step-by-step protocol.

rocessing multiple samples within a short time-window, e.g., after a physiological stimulus. In this chapter, we discuss some of the current methods to take tissue samples from frozen tissue, keeping in. mind the requirements for subcellular isolation (e.g., synaptosomes) of pro-teomics samples. In addition, we .

6 days of age, anesthesia can be achieved through hypothermia (Behringer et al., 2013). Place mouse pup in a conical tube or wrap it with latex glove (cut off the thumb of the glove for this purpose) and bury. it up to the neck in crushed ice for 10-15 min to obtain a surgical pane of anesthesia. Analg. sia state lasts ~10 min, thereby do not .

In this chapter, we discuss some of the current methods to take tissue samples from frozen tissue, keeping in mind the requirements for subcellular isolation (e.g., synaptosomes) of proteomics samples. In addition, we provide a step-by-step protocol to improve standardization of tissue dissection in your lab.