U.S. Environmental Protection Agency has approved 4-methylumbelliferyl-β-D-glucuronide Escherichia coli broth medium (EC-MUG) is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples.

2022年8月10日 · The substrate, 4-methylumbelliferyl-β-D-glucuronide (MUG), is both sensitive and selective for detection of β-glucuronidase activity. Hence, the MUG test in conjunction with oxidase, indole, and lactose fermentation can be performed to effectively identify E. coli and related organisms.

Approved by the U.S. Environmental Protection Agency (24), 4-methylumbelliferyl-β-D-glucuronideEscherichia coli broth medium (EC-MUG) is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples.

An assay procedure to screen for Escherichia coli in foods by using 4-methylumbelliferyl-beta-D-glucuronide (MUG) incorporated into lauryl tryptose (LST) broth was evaluated. The beta-glucuronidase produced by E. coli cleaves the MUG substrate to yield a fluorescent end product.

2024年5月10日 · Most strains of E. coli make an enzyme called β-D-glucuronidase. This enzyme cuts the substrate, 4-methylumbelliferyl—D-glucuronide (MUG), and makes methylumbelliferone, which is a fluorescent product. A source of long-wave ultraviolet light …

2022年1月3日 · Principle of MUG Test. MUG is an acronym for 4-methylumbelliferyl-beta-D-glucuronide, a fluorogenic substrate of β-glucuronidase. β-D-Glucuronidase is an enzyme produced by most strains of E. coli and other Enterobacteriaceae which hydrolyzes β-d-glucopyranosid-uronic derivatives to aglycons and d-glucuronic acid. The substrate 4 ...

The lauryl tryptose broth-MUG-most-probable-number assay was superior to violet red bile agar for the detection of heat- and chlorine-injured E. coli cells. Anaerogenic strains produced positive reactions, and small numbers of E. coli could be detected in the presence of large numbers of competing bacteria.

2010年8月1日 · EC broth and agar media with MUG are best suited for confirmatory testing of the presence of E. coli after a presumptive positive result for fecal coliform bacteria. In this protocol, we will emphasize using EC broth and EC agar media with MUG for E. …

A MUG- positive (E- coli) and MUG- negative (e.g., uninoculated) control are necessary for each analysis. The observation of bright blue fluorescence in the EC + MUG tubes after 24 + 2 hours constitutes apositive test for £. coli.

2020年6月5日 · Principle of MUG test: About 97% strains of Escherichia coli and organisms as such Salmonella, Shigella, and Yersinia are capable of producing enzyme β-glucuronidase. MUG stands for 4-Methylumbelliferyl-β-D Glucuronide which is a …