Vector for fusing EGFP to the C-terminus of a partner protein. For other reading frames, use pEGFP-N2 or pEGFP-N3. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Sticky ends from different AflIII sites may not be compatible. PciI is inhibited by nonionic detergents.

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

Plasmid pEGFP-N1-TFEB from Dr. Shawn Ferguson's lab contains the insert TFEB and is published in Sci Signal. 2012 Jun 12;5 (228):ra42. This plasmid is available through Addgene.

pEGFP-N1编码野生型GFP（1-3）的红移变体，该变体已经过优化，可以在哺乳动物细胞中获得更亮的荧光和更高的表达。 （最大激发波长＝ 488nm；最大发射波长＝ 507nm。 ）pEGFP-N1编码GFPmut1变体（4），其包含Phe-64对Leu和Ser-65对Thr的双氨基酸取代。 EGFP基因的编码序列包含超过190个沉默碱基的变化，这些变化对应于人类密码子使用偏好（5）。 EGFP侧翼的序列已转化为Kozak共有翻译起始位点（6），以进一步提高真核细胞的翻译效率。 pEGFP-N1中 …

The pEGFP-N1 is a vector for fusing EGFP to the C-terminus of a partner protein. It has multiple cloning sites before the GFP gene.

提供 pAcGFP1-N1 载体/质粒图谱，全长序列，抗性，大小和元件信息。

pEGFP-N1-FLAG质粒价格1200元，pEGFP-N1-FLAG(addgene 60360 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为卡那霉素，质粒大小为4733 bp，测序引物为CMV Forward/EGFP-N。

2011年9月22日 · 与以往常用的报告基因相比，gfp具有以下优点： ①在荧光显微镜下，用波长约490nm的紫外线激发后，即可观察到绿色荧光，直接、简捷、便于检测；

2022年1月7日 · 与以往常用的报告基因相比，gfp具有以下优点： ①在荧光显微镜下，用波长约490 nm的紫外线激发后，即可观察到绿色荧光，直接、简捷、便于检测；