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Methods used for the measurement of blood-brain barrier integrity
2021年2月26日 · Sodium fluorescein (NaF) has been widely used in the evaluation of BBB permeability (He et al. 2014; Lassmann et al. 1984; Roszkowski and Bohacek 2016). NaF is …
Sigma Fluorescein sodium 荧光素钠盐(血脑屏障通透性测定)
体外血脑屏障(bbb)模型中最常用方法是使用静脉注射的活体染料(示踪剂,探针),分子量大于180da,可排除在外不能穿透完整脑屏障。 满足此要求最小分子量探针是荧光素钠 …
【求助】荧光素钠测定血脑屏障通透性 - DXY.cn
2012年6月25日 · 各位前辈:本人实验中采用荧光素钠(FLUORESCEIN SODIUM )测定血脑屏障通透性,具体方法如下:小鼠腹腔注射1%NaFI(5ml/kg),一小时后灌注取脑,然后1ml PBS …
Temporal and Spatial Effects of Blast Overpressure on Blood-Brain ...
2018年6月6日 · Extravasation of sodium fluorescein (NaF) and Evans blue (EB) tracers were used to demonstrate that compromise of the BBB occurs immediately following shock loading, …
Human iPSC-derived brain endothelial microvessels in a multi-well ...
2022年7月1日 · Due to its molecular properties, NaF has been suggested as the tracer of choice that enables the detection of small changes in BBB tightness associated to small …
Brain metastatic cancer cells release microRNA-181c-containing
2015年4月1日 · Here we show that cancer-derived extracellular vesicles (EVs), mediators of cell–cell communication via delivery of proteins and microRNAs (miRNAs), trigger the …
Characterization of the blood–brain barrier in genetically …
2021年7月28日 · In this study, we characterized the BBB of eight inbred mouse strains; A/J, C57BL/6 J, CAST/EiJ, DBA/2J, NOD/ShiLtJ, PWK/PhJ, WSB/EiJ, and 129S1/SvImJ using …
使用血管通透性标记物评估血脑屏障完整性:伊文思蓝、荧光素钠 …
由脑微血管内皮细胞构成的血脑屏障(BBB)是一个动态界面,它控制和调节包括肽、蛋白质、离子、维生素、激素和免疫细胞在内的各种物质从循环进入脑实质的运输. 某些疾病/病症,如阿 …
伊文思蓝和荧光素钠对大鼠脑外渗的不同模式。,PLOS ONE - X-MOL
2013年7月5日 · 埃文斯蓝染料 (EBD; 961 Da) 和荧光素钠染料 (NaF; 376 Da) 是血脑屏障 (BBB) 研究中常用的惰性示踪剂。 它们都是具有相似疏脂特性的高电荷低分子量 (LMW) 示踪剂。