实验原理：氮蓝四唑 (NBT, nitroblue tetrazolium) 与超氧阴离子发生反应并形成蓝色的 甲臢 (formazan)。 实验目的：定性、原位检测水稻各组织中的超氧阴离子(O 2- )。

2018年10月3日 · NBT staining is performed on tissue sections cut between 10–12 microns thick. Histologically, the thermally damaged/injured tissue is quantified by assessing the extent of NBT-negative (yellow-tan in color) staining within the treatment zone.

Nitro blue tetrazolium is a chemical compound composed of two tetrazole moieties. It is used in immunology for sensitive detection of alkaline phosphatase (with BCIP). NBT serves as the oxidant and BCIP is the AP-substrate (and gives also dark blue dye).

Nitro blue tetrazolium is a widely used blue stain for NADPH diaphorase activity, and it has been observed that staining correlates well with NOS activity. The NOS contribution to the staining cannot be quantified, as several enzymes show diaphorase activity.

2020年8月4日 · This study aimed to develop an alternative method for detecting and quantifying intracellular ROS in oocytes, cumulus cells and embryos based on nitroblue tetrazolium (NBT) staining and bright-field microscopy.

This study aimed to develop an alternative method for detecting and quantifying intracellular ROS in oocytes, cumulus cells and embryos based on nitroblue tetrazolium (NBT) staining and bright-field microscopy.

Superoxide anion is detected with nitroblue tetrazolium (NBT) and hydrogen peroxide by Diaminobenzidine tetrahydrochloride (DAB) staining. In this chapter, methods for determining total ROS and lipid peroxidation assay, histochemical staining techniques for superoxide and H(2)O(2) molecules are described.

Nitro blue tetrazolium chloride (NBT) can be used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

The results of combining a particular counterstain with any of the mounting media primarily depends on the type of tissue used for NBT/BCIP color detection. Stain adjacent slides with or without NBT/BCIP detection with a typical counterstain and mount the slides with the classical xylene containing mounting medium: This allows the direct ...

nbt可作为一种筛查 尿道感染 的方法。 氯化硝基四氮唑蓝（NBT）还原法对血清糖化低密度脂蛋白及 极低密度脂蛋白 （SG LP-V P）进行测定，依据呈色的深浅直接用光密度表示SG LP-V P的浓度，并计算SG LP-V P的指数，取得的效果非常好。