HLA typing by next-generation sequencing (NGS) generates unambiguous, phase-resolved HLA typing results using a single assay, system, and analysis program. The HLA region is the most densely polymorphic region of the genome.

随着二代测序技术的不断发展，基于 ngs 的hla分型中应运而生。它具有通量高、实验周期短、高分辨率和成本低等特点，本篇主要介绍利用生物信息的手段对ngs的数据进行hla分型。

2020年6月14日 · HLA (human leukocyte antigen ，人类白细胞抗原)是人类的主要组织相容性复合体（MHC）的表达产物，该系统是所知人体最复杂的多态系统。 HLA是具有高度多态性的同种异体抗原，其化学本质为一类糖蛋白，由一条α重链（被糖基化的）和一条β轻链非共价结合而成。 其肽链的氨基端向外（约占整个分子的3/4），羧基端穿入细胞质，中间疏水部分在胞膜中。 HLA按其分布和功能分为Ⅰ类抗原和Ⅱ类抗原。 HLA-I类分子：内源性抗原的递呈分子， HLA-Ⅱ类 …

2015年8月27日 · Research on the human leukocyte antigen (HLA), an extensively studied molecule involved in immunity, has benefitted from NGS technologies. The HLA region, a 3.6-Mb...

Here, we introduce the first highly automated open-kit/open-source HLA-typing method for NGS. The method employs in-solution targeted capturing of the classical class I (HLA-A, HLA-B, HLA-C) and class II HLA genes (HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1).

This brief review will summarize the utility of next-generation sequencing (NGS) for HLA genotyping, highlighting the advantages of this approach over other molecular methods for typing HLA alleles. To accurately determine an HLA genotype, phase (cis/trans) must be established between polymorphic positions both within individual exons and ...

2015年6月23日 · High-quality NGS-based methods are in proprietary use and not publicly available. Here, we introduce the first highly automated open-kit/open-source HLA-typing method for NGS. The method employs in-solution targeted capturing of the classical class I (HLA-A, HLA-B, HLA-C) and class II HLA genes (HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1).

2025年1月15日 · Next-generation sequencing (NGS) is revolutionizing HLA gene analysis with its ability to identify allelic variants (haplotypes) and rare polymorphisms. Unlike traditional methods, which rely on specific probes or targeted sequences, NGS uses massively parallel sequencing to generate comprehensive data, including intronic and non-coding regions ...

2023年2月22日 · Next-generation sequencing (NGS) has revolutionized clinical genotyping, providing high-resolution HLA genotyping with a low ambiguity rate. This study aimed to develop new NGS-based HLA genotyping (HLAaccuTest, NGeneBio, Seoul, KOREA) on the Illumina MiSeq platform and validate the clinical performance.

The advent of next generation sequencing (NGS) has altered the face of genotyping the human leukocyte antigen (HLA) system in clinical, stem cell donor registry, and research contexts. NGS has led to a dramatically increased sequencing throughput at high accuracy, while being more time and cost efficient than precursor technologies.