2020年10月10日 · Stronger cGMP signals, that can inhibit phosphodiesterase enzymes, allowed [cAMP] to rise, as demonstrated by a FRET-based sensor. Inferring from the actions of membrane-permeant analogues, cGMP was determined to activate NHE1, whereas cAMP was inhibitory, which explains the biphasic regulation by NO.

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, …

The discovery of the capacity of NO to fine-tune NHE1 activation via biphasic effects significantly increases our understanding of the complexity and flexibility of pH regulation in cardiomyocytes and the complex effects of NO on cardiac physiology.

2022年8月15日 · Isoform one (NHE1) is present on the plasma membrane and regulates intracellular pH by removal of one intracellular proton in exchange for one extracellular sodium thus functioning as an electroneutral process. Human NHE1 has a 500 amino acid membrane domain plus a C-terminal 315 amino acid, regulatory cytosolic tail.

2021年2月21日 · This mini review focuses on the ubiquitous isoform 1 (NHE1) of this protein family also known as the “cardiac isoform” and its regulation by the cyclic guanosine monophosphate/protein kinase G (cGMP/PKG) signaling route, triggered by inhibition of the phosphodiesterase 5A (PDE5A) by Sildenafil.

67363-1-Ig targets NHE1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

2008年10月10日 · It is becoming apparent that NHE1 in the cardiac cell can be both stimulated and inhibited directly by intracellular signaling cascades. Like Dr Dolittle, these talk to the transporter, and the chorus determines whether cellular H + extrusion during intracellular acidosis is stimulated or inhibited.

N-terminal由12个α螺旋跨膜结构域构成，行使NHE1的主要职责既通过等比例对换胞内的氢离子和胞外的钠离子来调节细胞内的酸碱度（pHi），该区域也被认为是NHE1抑制剂的结合区 [21]。

2017年9月3日 · 这类 NHEs存在于大多数细胞 中，主 次命名为 NHE1～NHE9，具有不同的组织分布和 要分布于分泌性内膜 (如高尔基体扁平囊，反式高尔 功能特性。 近年来研究表 明，NHE除能调节细胞 基体管网状结构和分泌囊泡)及 内吞 ／降解系统 (如 内的pH值外，还参与了上皮细胞的盐运输和细胞 再循环内体 ，晚期核内体和溶酶体)。 内膜型NHEs 体积 的调节等生理过程 ，与一些疾病的发生发展密 不仅转 运细胞外 的 Na 和 Ii，还 可 以转 运 K 。 切相关 。 本文将对 NHE …

从机制上，我们首次发现葛根素可抑制心力衰竭中过度激活的Na +/H + 交换异构体1（NHE1），从而可能通过降低Na + 和Ca 2+ 离子浓度以及减轻钙超载引起的线粒体损伤来改善心力衰竭；另一方面，葛根素抑制心衰时p38通路的激活，减少TGF-β和促炎细胞因子的表达 ...