2021年5月22日 · MP NLS are a single cluster composed of 4–8 basic amino acids, which generally contains 4 or more positively charged residues, that is, arginine (R) or lysine (K). The characteristic motif of MP NLS is usually defined as K (K/R) X (K/R), where X can be any residue .

A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport. [1] Typically, this signal consists of one or more short sequences of positively charged lysines or arginines exposed on the protein surface. [1] Different nuclear localized proteins may share ...

NLSdb is a database collecting nuclear export signals (NES) and nuclear localization signals (NLS) along with experimentally annotated nuclear and non-nuclear proteins. NES and NLS are short sequence motifs related to protein transport out of and into the nucleus. The updated NLSdb now contains 2253 NLS and introduces 398 NES.

2024年9月9日 · We also reveal the functional role of the NLS‐coupled NoLS motif in driving nucleolar localization of NML, which contains an arginine‐rich motif essential for its function.

NLSs are known to have diverse patterns, of which only a limited number are covered by currently known NLS motifs. Here we propose a sequential pattern mining algorithm SeqNLS to effectively identify potential NLS patterns without being constrained by the limitation of …

2006年8月11日 · Further analyses reveal three rules for NLS recognition by Kapβ2: NLSs are structurally disordered in free substrates, have overall basic character, and possess a central hydrophobic or basic motif followed by a C-terminal R/H/KX (2-5) PY consensus sequence. We demonstrate the predictive nature of these rules by identifying NLSs in seven ...

1 天前 · The refined NLS motif is marked by the red bar. Dark shading, identical residues; light shading, similar residues. * represents the identical animo acids between two sequences. ( B ) Point mutations of all the NLSs (MS188 12–15 , MS188 18–22 , and MS188 96–107 ) in MS188, TDF1, and MYB2 were constructed and fused with GFP and then ...

2 天之前 · The Nuclear Localization Signal (NLS) is the recognition motif found in cargo proteins that allows them to attach to importins [8,9]. Until recently, only a few NLS motifs for importin α (IMPOα) and the M9 NLS (recognized by importin β2, also known as transportin) have been identified, despite significant scientific efforts [ 10 , 11 , 12 ...

A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins into the nucleus (Figure 1). NLS motifs play a key role in this mechanism; (i) typically, deletion of the NLS disrupts nuclear import; and (ii) frequently, a non-nuclear protein will be imported into the nucleus if fused to an NLS.

2025年2月26日 · Our earlier observations showed that TAZ/YAP harbour a C-terminal, unconventional nuclear localization signal (NLS). Here, we show that this sequence, necessary and sufficient for basal, ATP-independent nuclear import, contains an indispensable central methionine flanked by negatively charged residues.