2023年5月18日 · Here, we demonstrate that lncRNA LoNA tethers granular-component-enriched NPM1 to dense-fibrillar-component-enriched FBL and drives the formation of compartmentalized nucleolus via facilitating...

FBL是rRNA前体加工过程中的一种重要的加工因子，包含了一个富含甘氨酸和精氨酸的内在无序区域（intrinsically disordered regions，IDRs）结构域（GAR）以及一个甲基转移酶结构域（MD）。

2024年5月14日 · EGFP-tagged proteins (FBL, MD, GAR, NPM1 and PLD-MD) were cloned into pET-28a for expression in Escherichia coli BL21 (DE3) (AlpalifeBio KTSM104L), induced with 0.5 mM isopropyl β-d-1 ...

2023年6月21日 · In this study, we demonstrated that the nucleolar protein ZNF692 directly interacts with nucleophosmin 1 (NPM1). Knocking down ZNF692 resulted in the nucleolar redistribution of NPM1 in ring-like structures and reduced protein synthesis.

2019年8月9日 · Two key nucleolar proteins that have received the most attention are nucleophosmin (B23/NPM1) and fibrillarin (FBL). NPM1 is a highly expressed nucleolar protein that is particularly enriched in the granular component (GC), the outer layer of the nucleolus that is associated with assembling ribosomal proteins together with processed rRNA into ...

2023年5月18日，中国科学技术大学刘强团队在Nature Structural Molecular Biology杂志上在线发表了题为Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment...

2019年9月30日 · These NPM1 mutations inhibit ribosomal RNA 2´-O-methylation by decreasing the binding of the rRNA methyltransferase FBL to small nucleolar RNAs. Thus, NPM1 influences ribosomal functions via...

2024年8月21日 · We focused on a cellular chromatin remodeling protein, NPM1, which plays a crucial role in nucleolar formation through LLPS. NPM1 facilitates LLPS by interacting with adenovirus protein VII, effectively accumulating protein VII into membrane-less compartments called virus-induced post-replication bodies.

Here, we demonstrate that lncRNA LoNA tethers granular-component-enriched NPM1 to dense-fibrillar-component-enriched FBL and drives the formation of compartmentalized nucleolus via facilitating liquid–liquid phase separation of those two nucleolar proteins. Phenotypically, LoNA-deficient embryos show developmental arrest at the two-cell (2C) stage.

2023年5月18日 · Here, we demonstrate that lncRNA LoNA tethers granular-component-enriched NPM1 to dense-fibrillar-component-enriched FBL and drives the formation of compartmentalized nucleolus via facilitating liquid-liquid phase separation of those two nucleolar proteins. Phenotypically, LoNA-deficient embryos show developmental arrest at the two-cell (2C) stage.