Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work …

PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe . Cipakova I et al. Cell Cycle 2019 …

2025年1月20日 · Here, we report two cryo-electron microscopy structures of post-B act spliceosome intermediates from Schizosaccharomyces pombe primed for disassembly. We …

2017年9月21日 · In this paper, we report the cryo-EM structure of the S. cerevisiae ILS complex at an average resolution of 3.5 Å, which allows identification of the disassembly proteins …

Although S. pombe contains no clear Ntr2 homolog, it is of note that Ntr1, Prp43, and Brr2 were all significantly substoichiometric in our purified complexes (Table 1). During spliceosome …

2019年6月20日 · Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. …

pombe. Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This …

2022年3月18日 · Here, we present a protocol for tandem affinity purification (TAP) of protein complexes from the fission yeast Schizosaccharomyces pombe. The protocol employs cells …

Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work …

The recruitment of Ntr2 and Ntr1 into the spliceosome serves two purposes: to ensure the correct spliceosomal complex is chosen for disassembly and to activate the helicase Prp43. The …