2018年10月19日 · Chang教授及其同事首次开发了可以在活细胞中全面分析RNA二级结构的新技术，SHAPE（selective 2'-hydroxyl acylation and profiling experiment）。 SHAPE分析首先是用NAI-N3修饰未配对的核苷酸，然后让生物素与NAI-N3结合，通过链霉亲和素磁珠回收修饰过的RNA。

体内shape试剂用于rna结构的测定。该试剂含有高纯度形式的2-甲基烟酸咪唑化物（nai）。该化合物在rna的单链区域上形成2’-o-加合物，可以通过选择性2’-羟基酰化和引物延伸（shape）进行检测。

In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This compound forms 2’-O-adducts on single stranded regions of RNA that can be detected by Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).

2012年11月25日 · Here we design, synthesize and demonstrate two new chemical probes that enable selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human,...

2016年1月14日 · In brief, the bifunctional NAI-N 3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at...

2021年12月1日 · NAI is used as the SHAPE reagent to probe circular RNA secondary structures in cells. Experimental and unique computational pipelines are required for circular RNA secondary structural prediction. Circular RNAs are produced from back-splicing of exons of precursor mRNAs (pre-mRNAs).

Here, we introduce a SHAPE Selection (SHAPES) reagent, N-propanone isatoic anhydride (NPIA), which retains the ability of SHAPE reagents to accurately probe RNA structure, but also allows covalent coupling between the SHAPES reagent and a biotin mol.

NAI-N3 is a dual-function SHAPE (selective 2-hydroxyl acylation and profiling experiment) probe (RNA structure probe and enrichment). It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups.

2018年5月3日 · We detail several strategies by which in-cell SHAPE-MaP can inform new biological hypotheses and emphasize downstream analyses that reveal sequence or structure motifs important for RNA ...

Recent advances in SHAPE technology have converted the classic primer extension method to next-generation sequencing platforms, allowing transcriptome-level analysis of RNA secondary structure. In particular, icSHAPE and SHAPE-MaP, using NAI-N3 and ...