2014年8月20日 · Treatment of cultured human umbilical vein endothelial cells (HUVEC) with visfatin/Nampt increased the fraction of cells staining positive for senescence-associated-β-galactosidase (SA-β-gal), a marker of cell senescence [5], in a concentration-dependent manner, with 50 ng/ml being the lowest concentration tested that caused a significant ...

2022年10月5日 · Here we show that ionizing radiation evokes NAMPT activation within 30 min without apparent changes in its protein expression. AMPK rapidly phosphorylates NAMPT at S314 under ionizing radiation, which reinforces the enzymatic activity of NAMPT by increasing NAMPT binding with its substrate phosphoribosyl pyrophosphate (PRPP).

In HUVEC, visfatin/Nampt activates the inflammation-related transcription factor NF-κB [82, 84, 97] and promotes the expression of cell adhesion molecules, such as ICAM-1, VCAM-1, or E-selectin [84, 97], as key molecules implicated in leukocyte recruitment and early proatherosclerotic events .

2020年3月25日 · In human umbilical endothelial cells (HUVEC), proteins related to the NLRP3-inflammasome phosphorylated p-65, NLRP3, caspase-1, pro-IL-1β, and mature IL-1β were determined by Western blot, while...

Nampt, HUVEC exhibited higher p53 levels (Fig. 2D), in line with the concept that stabilization of this protein is a key downstream signaling event mediating the response to DNA damage and telomere dysfunction

2020年3月25日 · In human umbilical endothelial cells (HUVEC), proteins related to the NLRP3-inflammasome phosphorylated p-65, NLRP3, caspase-1, pro-IL-1β, and mature IL-1β were determined by Western blot, while the inflammasome related apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC-specks) was studied by immunofluorescence.

2025年3月1日 · S. chinensis ameliorated the downregulation of NAD+ levels in HUVEC s caused by oxygen/glucose deprivation, so we investigated the mechanism and screened the active components in S. chinensis by spectrum-effect correlation analysis and target cell extraction.

2011年11月3日 · Visfatin impairs the relaxation induced by BK in human mesenteric microvessels via Nampt activity. Human microvessels were pre-incubated with visfatin (50 ng/mL) and/or the Nampt inhibitor APO866 (10 µmol/mL), contracted with 35 mmol/L KCl and then exposed to increasing concentrations of BK (1 nmol/L to 3 µmol/L).

2014年5月1日 · Human umbilical vein endothelial cells (HUVEC) obtained at delivery from gestational diabetic women were used to study the transcriptomic effects of chronic hyperglycemia in fetal vascular cells using Affymetrix microarrays.

2024年5月5日 · Our experimental results showed that scavenging MitoROS reversed the young phenotype in KLF4 knockdown HUVECs, whereas NAMPT knockdown accelerated HUVEC senescence and the SASP phenotype in KLF4 knock-in HUVECs.