The advent of nano-electrospray ionization (nano-ESI) has considerably extended the usability of ESI in the analytical mass spectrometric laboratory. One of the remarkable features of nano-ESI is its extremely low sample consumption.

nano Electrospray ionization (ESI) produces ions from liquid samples. It is ideal for the analysis of large biomolecules, including proteins and lipids. A high voltage is applied to the liquid until coulombic repulsion overcomes surface tension. This produces tiny droplets highly charged.

2005年10月1日 · Nano-electrospray ionization (nano-ESI) is generally recognized as the most efficient method of introducing a liquid sample for direct analysis by mass spectrometry.

2022年11月1日 · With the assistance of auxiliary nitrogen gas flow, the ion funnel interface could efficiently deliver ions from each electrospray into the MS for analysis. Compared with a single ESI implemented on the interface, the total ion current transmitted through the MS and the signal-to-noise ratio increased 3.8 times using four ESI inlets.

2023年3月23日 · Owing to the smaller primary droplets, nano-electrospray can remarkably improve desolvation, ionization and ion transmission efficiencies, compared with conventional ESI sources 9,96.

2024年4月25日 · Here we introduce ESI-cryoPrep, a specimen preparation method based on electrospray ionization in native mass spectrometry, designed to alleviate issues associated with protein denaturation or...

2023年10月10日 · Nanoelectrospray ionization (nano-ESI) is a highly efficient and a widely used technique for the ionization of minute amounts of analyte. Offline nano-ESI sources are convenient for the direct infusion of complex mixtures that suffer from high matrix content and are crucial for the native mass spectrometric analysis of proteins.

2024年4月9日 · This study explores the innovative field of pulsed direct current arc-induced nanoelectrospray ionization mass spectrometry (DCAI-nano-ESI-MS), which utilizes a low-temperature direct current (DC) arc to induce ESI during MS analyses.

2020年1月21日 · We demonstrate that a pulsed nano-ESI operated at 20 Hz with ion generation pulses of 170–510 μs offers reproducible ion arrival times (0.09–0.21% RSD). Arrival times are then translated to effective collision cross sections (CCSs) using tetraalkylammonium ions as CCS internal standards.

NanoESI, which refers to ESI performed at flow rates in the range of 200 to 1000 nL/min using smaller diameter emitters (10 to 100 microm id), produces smaller droplets than conventional ESI resulting in more efficient ionization.