2014年7月1日 · We observed that phosphorylation of Nanog by ERK1 decreases Nanog stability through ubiquitination-mediated protein degradation. Further, we found that this phosphorylation induces binding of FBXW8 with Nanog to reduce Nanog protein stability.

2024年7月15日 · We show that a primary consequence of ERK activation in mouse embryonic stem cells is elimination of Nanog, which precipitates breakdown of the naïve state gene regulatory network. Variability in pERK dynamics results in heterogeneous loss of Nanog and metachronous state transition.

2020年6月23日 · We begin with a brief overview of the structure, regulation, substrate recognition and subcellular localization of ERK1 and ERK2. We then systematically discuss how ERK signalling regulates six...

2022年9月6日 · We show that NANOG activates ERK signaling by inducing Fgfr2, Fgf4, and Fgfbp1. The activated ERK together with NONO represses transcription of Nanog, resulting in a NANOG‐pERK reciprocal regulatory loop (Fig 6E). The subpopulation of ES cells expressing high NANOG will have higher FGFR2.

2014年10月1日 · Experiments modulating the Fgfr/Erk pathway in Nanog mutants revealed that, in a first phase around E2.5, Gata6 expression is induced by the Erk pathway. Afterwards, Erk signaling progressively becomes dispensable for the maintenance of Gata6 expression in the absence of Nanog, but remains necessary to counteract the Nanog-induced Gata6 ...

Nanog regulates human and mouse embryonic stem (ES) cell self-renewal activity. Activation of ERKs signaling negatively regulates ES cell self-renewal and induces differentiation, but the mechanisms are not understood. We found that ERK1 binds and phosphorylates Nanog.

2022年11月7日 · Here, we show that a dose-dependent induction of Fgfbp1 and Fgfr2 by NANOG activates autocrine-mediated ERK signaling in Nanog-high cells to trigger autorepression. pERK recruits NONO to the Nanog locus to repress transcription by preventing POL2 loading.

2020年11月9日 · We present correlative evidence that: i) ERK activity is uniquely heterogeneous immediately following mitosis, ii) mitotic exit signaling correlates with NANOG and GATA6 expression, iii) NANOG protein is stabilized in a subset of ES cells after mitosis, and iv) ERK inhibitors promote NANOG stabilization specially after mitosis.

We demonstrate that NANOG is directly phosphorylated by ERK2 and CDK1 in vitro. We connect cell-cycle- and growth-factor-mediated signaling to NANOG. NANOG is a divergent homeobox protein and a core component of the transcriptional circuitry that …

2024年7月26日 · Here, we examine the role of mitogen-activated protein kinase (ERK1/2) signalling in pluripotent state transition. We show that a primary consequence of ERK activation in mouse embryonic stem cells is elimination of Nanog, which precipitates breakdown of the naïve state gene regulatory network.