An E. coli strain that carries the NcoI gene from Nocardia corallina (ATCC 19070). This product is related to the following categories: Restriction Endonucleases: N-O Products,

Thermo Scientific NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37C in Tango buffer (Isoschizomers: Bsp19I) . Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes

本酶提供高保真版本酶 NcoI-HF ® （NEB #R3193）。 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。

NcoI-HF® has the same specificity as NcoI (NEB #R0193), but it has been engineered for reduced star activity. Not sensitive to CpG, dcm, or dam methylation. Protocols, Manuals & Usage

Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds. Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site [5][6].

There are two KpnI sites in the SnapFast MCS. These sites allow the removal of the start codon within the NcoI site that they flank. This then allows downstream sites (e.g. EcoRV or XhoI) to be used instead to insert a gene, without the concern of premature ribosomal translation initiation at the start codon in the NcoI site.

Thermo Scientific NcoI 限制性内切酶可识别 C^CATGG 位点，于 37°C 下在 Tango 缓冲液中的切割效果最佳（同裂酶：Bsp19I）。 有关该酶和其他限制性内切酶的酶活性、双重酶切条件和热灭活的表格，请参见 限制性内切酶的反应条件 。

NcoI has a High Fidelity version NcoI-HF ® . High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Thermo Scientific FastDigest NcoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up ...

NcoI is a restriction endonuclease that is used in molecular biology applications to cleave the DNA recognition site 5′-C/CATGG-3′, resulting in DNA fragments with 5′-cohesive termini. It is derived from an E. coli strain with a cloned NcoI gene from Neisseria corrodens.