2023年12月15日 · Here, cryo-EM is used to study the inhibitor-bound human sodium-calcium exchanger NCX1.3, shedding light on the molecular mechanisms underlying SEA0400- and XIP-mediated inactivation. The XIP region is located within a groove between TMD and CBD2, mediating inactivation by obstructing the conformational transition of gating helices TMs 1/6.

2023年10月4日 · Here we present the cryo-EM structures of human cardiac NCX1 in both inactivated and activated states, elucidating key structural elements important for NCX ion exchange function and its...

2024年5月7日 · NCX1 activity is inhibited by intracellular Na + via a process known as Na + -dependent inactivation. A central question is whether this inactivation plays a physiological role in heart function....

摘 要：钠钙交换体(sodium calcium exchanger, NCX)是一种广泛分布于膜性结构(细胞质膜、线粒体膜、内质网、分泌小泡膜 等)上的阳离子转运蛋白，具有两种转运Ca 2+ 和Na + 的模式：介导Na + 内流、Ca 2+ 外排的前向模式(forward mode)和作用相反的

Our study indicated that P1 protein prevents NCX1 autoinhibition by binding to the XIP domain, thus leading to an increased activity of the exchanger. Furthermore, these results might lead to the development of peptidomimetic compounds that, by reproducing the stimulatory effect of the P1 peptide, might increase the activity of the exchanger.

2024年1月9日 · 这项研究通过冷冻电镜单颗粒技术重构出人源NCX1.3结合特异性抑制剂SEA0400的高分辨结构，揭示了XIP介导的自抑制机制和SEA0400的别构抑制机制。 钠钙交换蛋白（sodium-calcium exchange, NCX）属于SLC（Solute Carrier）家族和CaCA（Calcium/Cation Antiporter）超家族，是一种双向离子转运体，可以通过正向和反向转运方式，介导细胞内外Na离子和Ca 2+ 的交换，对于维持细胞内钙稳态发挥重要作用。 NCX参与体内多种生理过程，例 …

2024年1月8日 · 这项研究通过冷冻电镜单颗粒技术重构出人源NCX1.3结合特异性抑制剂SEA0400的高分辨结构，揭示了XIP介导的自抑制机制和SEA0400的别构抑制机制。 钠钙交换蛋白（sodium-calcium exchange, NCX）属于SLC（Solute Carrier）家族和CaCA（Calcium/Cation Antiporter）超家族，是一种双向离子转运体，可以通过正向和反向转运方式，介导细胞内外Na离子和 Ca 2+ 的交换，对于维持细胞内钙稳态发挥重要作用。 本网站所转载的文章，均来自互 …

2005年1月7日 · The sarcolemmal Na+-Ca2+ exchanger (NCX) is the main Ca2+ extrusion mechanism in cardiac myocytes and is thus essential for the regulation of Ca2+ homeostasis and contractile function. A cytosolic region (f-loop) of the protein mediates regulation of NCX function by intracellular factors including i …

The exchanger-inhibitory peptide (XIP) is trapped within a groove between the TMD and CBD2 and predicted to clash with gating helices TMs 1/6 at the outward-facing state, thus hindering conformational transition and promoting

2023年12月15日 · Here, cryo-EM is used to study the inhibitor-bound human sodium-calcium exchanger NCX1.3, shedding light on the molecular mechanisms underlying SEA0400- and XIP-mediated inactivation. The XIP region is located within a groove between TMD and CBD2, mediating inactivation by obstructing the conformational transition of gating helices TMs 1/6 .