2021年12月15日 · SpeI has a High Fidelity version SpeI-HF ® (NEB #R3133). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and …

Thermo Scientific BcuI (SpeI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in Tango buffer (isoschizomers: AhlI, SpeI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). Enjoy the enhanced performance and added value of our engineered ...

Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang.

High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

2018年7月30日 · 您可以通过我们的免费在线工具 NEBcloner ，来获取使用特定限制性内切酶进行单酶切反应的具体实验步骤。 请注意，NEBcloner 还将提供使用这种酶进行双酶切反应的详细步骤。 关于使用限制性内切酶进行酶切反应的更多信息，请参阅我们的参考文章：《优化限制性内切酶反应》。 资源链接 相关产品：ApaI、AccI、AflIII，更多+. View a general protocol for setting up a restriction digest; use our online tool to design specific protocols.

High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).

本酶提供高保真版 SpeI-HF ® （NEB #R3133）。 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。 HF 内切酶还会显著降低星号活性。 所有 HF 内切酶均符合省时酶（Time-Saver）标准，可在 5-15 分钟内酶切底物 DNA，也可实现过夜酶切而不会造成 DNA 降解。 HF 限制性内切酶在开发时便将性能作为重要指标，可在更宽的条件范围下具有完全活性，最大限度地减少非特异性酶切产物，同时为实验设 …

SpeI-HF ®. 我们很高兴地宣布，所有反应缓冲液均已不含 BSA（牛血清白蛋白）。从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白（rAlbumin）的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。如需更多信息，请访问 www.neb.cn/BSA-free。